Isolation of aquatic yeasts with the ability to neutralize acidic media, from an extremely acidic river near Japan's Kusatsu-Shirane Volcano

Autor: Masahiko Okai, Daisuke Mitsuya, Yu Wang, Naoto Urano, Masami Ishida, Takuya Hayashi, Mami Tanaka
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Molecular mechanism of neutralization of acid envirnments by alkalinization yeasts and their enzymes
Casamino acid
acid tolerance
Bioengineering
02 engineering and technology
Biology
Applied Microbiology and Biotechnology
Neutralization
03 medical and health sciences
chemistry.chemical_compound
acid-tolerant yeast
Candina fluviatilis
Japan
Rivers
Acid tolerance
0202 electrical engineering
electronic engineering
information engineering
Ammonium
Sulfate
Candida
chemistry.chemical_classification
Strain (chemistry)
アルカリ化酵母とその酵素系による強酸性水圏中和の分子機構の解明
acidic river
Hydrogen-Ion Concentration
neutralization
科学研究費研究成果
Yeast
Amino acid
030104 developmental biology
chemistry
Biochemistry
020201 artificial intelligence & image processing
Biotechnology
Nuclear chemistry
Zdroj: Journal of bioscience and bioengineering. 124(1):43-46
ISSN: 1347-4421
DOI: 10.1016/j.jbiosc.2017.02.005
Popis: The Yukawa River is an extremely acidic river whose waters on the east foot of the Kusatu-Shirane Volcano (in Gunma Prefecture, Japan) contain sulfate ions. Here we isolated many acid-tolerant yeasts from the Yukawa River, and some of them neutralized an acidic R2A medium containing casamino acid. Candida fluviatilis strain CeA16 had the strongest acid tolerance and neutralizing activity against the acidic medium. To clarify these phenomena, we performed neutralization tests with strain CeA16 using casamino acid, a mixture of amino acids, and 17 single amino acid solutions adjusted to pH 3.0, respectively. Strain CeA16 neutralized not only acidic casamino acid and the mixture of amino acids but also some of the acidic single amino acid solutions. Seven amino acids were strongly decomposed by strain CeA16 and simultaneously released ammonium ions. These results suggest strain CeA16 is a potential yeast as a new tool to neutralize acidic environments.
Databáze: OpenAIRE
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