Massive crossover elevation via combination of HEI10 and recq4a recq4b during Arabidopsis meiosis
| Autor: | Heïdi Serra, Catherine H Griffin, Raphael Mercier, Ian R. Henderson, Divyashree C Nageswaran, Mathilde Seguela-Arnaud, Christophe Lambing, Piotr Ziółkowski, Charles J Underwood, Stephanie D. Topp, Joiselle Blanche Fernandes |
|---|---|
| Přispěvatelé: | Department of Plant Sciences, University of Cambridge [UK] (CAM), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris Saclay (COmUE), Université Paris-Sud - Paris 11 (UP11), Biotechnology and Biological Sciences Research Council (BBSRC) [BB/L006847/1], BBSRC-Meiogenix IPA [BB/N007557/1], European Research Area Network for Coodinating Action in Plant Sciences/BBSRC 'DeCOP' [BB/M004937/1], European Research Council (SynthHotspot CoG), Gatsby Charitable Foundation [GAT2962], Royal Society University Research Fellowship, Bettencourt Schueller Foundation, European Project: 606956,EC:FP7:PEOPLE,FP7-PEOPLE-2013-ITN,COMREC(2013) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chromosomal Proteins Non-Histone Heterochromatin Arabidopsis Genetic recombination 03 medical and health sciences Meiosis Centromere Homologous chromosome meiosis RECQ4 [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Crossing Over Genetic Homologous Recombination 2. Zero hunger Genetics crossover Multidisciplinary biology Arabidopsis Proteins fungi DNA Helicases HEI10 Helicase Biological Sciences DNA Methylation Subtelomere recombination 030104 developmental biology biology.protein Homologous recombination |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2018, 115 (10), pp.2437-2442. ⟨10.1073/pnas.1713071115⟩ Proceedings of the National Academy of Sciences of the United States of America, 2018, 115 (10), pp.2437-2442. ⟨10.1073/pnas.1713071115⟩ Proceedings of the National Academy of Sciences |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1713071115 |
| Popis: | International audience; During meiosis, homologous chromosomes undergo reciprocal crossovers, which generate genetic diversity and underpin classical crop improvement. Meiotic recombination initiates from DNA double-strand breaks (DSBs), which are processed into single-stranded DNA that can invade a homologous chromosome. The resulting joint molecules can ultimately be resolved as crossovers. In Arabidopsis, competing pathways balance the repair of ∼100-200 meiotic DSBs into ∼10 crossovers per meiosis, with the excess DSBs repaired as noncrossovers. To bias DSB repair toward crossovers, we simultaneously increased dosage of the procrossover E3 ligase gene HEI10 and introduced mutations in the anticrossovers helicase genes RECQ4A and RECQ4B. As HEI10 and recq4a recq4b increase interfering and noninterfering crossover pathways, respectively, they combine additively to yield a massive meiotic recombination increase. Interestingly, we also show that increased HEI10 dosage increases crossover coincidence, which indicates an effect on interference. We also show that patterns of interhomolog polymorphism and heterochromatin drive recombination increases distally towards the sub-telomeres in both HEI10 and recq4a recq4b backgrounds, while the centromeres remain crossover suppressed. These results provide a genetic framework for engineering meiotic recombination landscapes in plant genomes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|