Novel Platform of Cardiomyocyte Culture and Coculture via Fibroblast-Derived Matrix-Coupled Aligned Electrospun Nanofiber
| Autor: | Seung Ja Oh, Chong-Hyun Kim, Ramesh Subbiah, Su-Hyun Kim, Kwideok Park, Sang-Heon Kim, Muhammad Suhaeri |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Scaffold Materials science Polyesters Nanofibers Nanotechnology 02 engineering and technology Matrix (biology) Extracellular matrix 03 medical and health sciences Tissue engineering medicine Animals Myocytes Cardiac General Materials Science Viability assay Fibroblast Cells Cultured Decellularization Tissue Engineering Tissue Scaffolds biology Fibroblasts 021001 nanoscience & nanotechnology Coculture Techniques Rats Fibronectin 030104 developmental biology medicine.anatomical_structure biology.protein 0210 nano-technology Biomedical engineering |
| Zdroj: | ACS Applied Materials & Interfaces. 9:224-235 |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.6b14020 |
| Popis: | For cardiac tissue engineering, much attention has been given to the artificial cardiac microenvironment in which anisotropic design of scaffold and extracellular matrix (ECM) are the major cues. Here we propose poly(l-lactide-co-caprolactone) and fibroblast-derived ECM (PLCL/FDM), a hybrid scaffold that combines aligned electrospun PLCL fibers and FDM. Fibroblasts were grown on the PLCL fibers for 5-7 days and subsequently decellularized to produce PLCL/FDM. Various analyses confirmed aligned, FDM-deposited PLCL fibers. Compared to fibronectin (FN)-coated electrospun PLCL fibers (control), H9c2 cardiomyoblast differentiation was significantly effective, and neonatal rat cardiomyocyte (CM) phenotype and maturation was improved on PLCL/FDM. Moreover, a coculture platform was created using multilayer PLCL/FDM in which two different cells make indirect or direct cell-cell contacts. Such coculture platforms demonstrate their feasibility in terms of higher cell viability, efficiency of target cell harvest (95% in noncontact; 85% in contact mode), and molecular diffusion through the PLCL/FDM layer. Coculture of primary CMs and fibroblasts exhibited much better CM phenotype and improvement of CM maturity upon either direct or indirect interactions, compared to the conventional coculture systems (transwell insert and tissue culture plate (TCP)). Taken together, our platform should be very useful and have significant contributions in investigating some scientific or practical issues of crosstalks between multiple cell types. |
| Databáze: | OpenAIRE |
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