Effect of luteotrophic hormone (LH) and thymosin on mitogen-induced blast transformation of Balb/c mouse lymphoid cells
| Autor: | M. Rouabhia, P. Deschaux, Z. Ghanmi, J. Chakir, O. Othmane |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
BALB/c Mouse medicine.drug_class chemical and pharmacologic phenomena Stimulation Biology Peptide hormone Lymphocyte Activation General Biochemistry Genetics and Molecular Biology Mice In vivo Internal medicine medicine Splenocyte Animals Humans General Pharmacology Toxicology and Pharmaceutics Cells Cultured Mice Inbred BALB C Cell growth Thymosin General Medicine Luteinizing Hormone Endocrinology Gonadotropin Mitogens Spleen |
| Zdroj: | Life sciences. 46(4) |
| ISSN: | 0024-3205 |
| Popis: | The present study investigates the effect of a pituitary hormone (LH) and a thymic factor (Thymosin Fraction 5 = TF5) on in vitro and in vivo spleen cell proliferative response of 2, 6 and 8-month-old Balb/c mice. In vitro experiments showed that the addition of LH at various concentrations (0.5, 5, 50 or 500 ng/ml) to cultures increased significantly the proliferative response to some known mitogens (ConA, PHA, PWM). The LH stimulation was further enhanced by subsequent addition of TF5 to these cultures. However, the addition of LH to LPS cultures resulted in a blockage of the cell growth which persisted after the addition of TF5. In vivo experiments showed that injection of LH (5 or 50 ng/ml) to 2, 6 and 8-month-old Balb/c mice had a significant increase on blast transformation of lymphoid cells following their incubation with ConA, PHA and PWM, and a significant decrease when they were incubated with LPS. The physiological significance of these findings which point out an intimate connection between immune and endocrine functions is discussed. |
| Databáze: | OpenAIRE |
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