Sensitive and fast identification of bacteria in blood samples by immunoaffinity mass spectrometry for quick BSI diagnosis
| Autor: | Alexandra Bondarenko, Pengyuan Yang, Niels Lion, Zhuoxin Chen, Hubert H. Girault, Lysiane Tissières Lovey, Natalia Gasilova, Michel Prudent, Horst Pick, Tianqi Gong, Siham Beggah Möller, Liang Qiao, Yingdi Zhu |
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| Rok vydání: | 2016 |
| Předmět: |
Chromatography
02 engineering and technology General Chemistry Biology 010402 general chemistry 021001 nanoscience & nanotechnology Mass spectrometry medicine.disease_cause biology.organism_classification 01 natural sciences 0104 chemical sciences Blood serum Staphylococcus aureus Clinical diagnosis Long period medicine 0210 nano-technology Escherichia coli Bacteria Whole blood |
| Zdroj: | Chemical Science, vol. 7, no. 5, pp. 2897-2995 |
| ISSN: | 2041-6539 2041-6520 |
| Popis: | Bloodstream infections rank among the most serious causes of morbidity and mortality in hospitalized patients, partly due to the long period (up to one week) required for clinical diagnosis. In this work, we have developed a sensitive method to quickly and accurately identify bacteria in human blood samples by combining optimized matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) and efficient immunoaffinity enrichment/separation. A library of bacteria reference mass spectra at different cell numbers was firstly built. Due to a reduced sample spot size, the reference spectra could be obtained from as few as 10 to 10(2) intact bacterial cells. Bacteria in human blood samples were then extracted using antibodies-modified magnetic beads for MS fingerprinting. By comparing the sample spectra with the reference spectra based on a cosine correlation, bacteria with concentrations as low as 500 cells per mL in blood serum and 8000 cells per mL in whole blood were identified. The proposed method was further applied to positive clinical blood cultures (BCs) provided by a local hospital, where Escherichia coli and Staphylococcus aureus were identified. Because of the method's high sensitivity, the BC time required for diagnosis can be greatly reduced. As a proof of concept, whole blood spiked with a low initial concentration (10(2) or 10(3) cells per mL) of bacteria was cultured in commercial BC bottles and analysed by the developed method after different BC times. Bacteria were successfully identified after 4 hours of BC. Therefore, an entire diagnostic process could be accurately accomplished within half a day using the newly developed method, which could facilitate the timely determination of appropriate anti-bacterial therapy and decrease the risk of mortality from bloodstream infections. |
| Databáze: | OpenAIRE |
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