In vitro toxicological assessment of flumethrin’s effects on MCF-7 breast cancer cells
Autor: | S Kara-Ertekin, Melike Erkan, S Yazar |
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Rok vydání: | 2021 |
Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
0301 basic medicine Cell Survival Health Toxicology and Mutagenesis Apoptosis Breast Neoplasms Endocrine Disruptors 010501 environmental sciences Toxicology medicine.disease_cause Flumethrin 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Pyrethrins Progesterone receptor medicine Estrogen Receptor beta Humans Cytotoxicity 0105 earth and related environmental sciences Chemistry Cell growth Estrogen Receptor alpha Estrogens General Medicine Molecular biology Gene Expression Regulation Neoplastic Comet assay 030104 developmental biology Proto-Oncogene Proteins c-bcl-2 MCF-7 MCF-7 Cells Female Trefoil Factor-1 Tumor Suppressor Protein p53 Genotoxicity DNA Damage Mutagens |
Zdroj: | Human & Experimental Toxicology. 40:2165-2177 |
ISSN: | 1477-0903 0960-3271 |
Popis: | Pyrethroid pesticides are frequently used for household insect control of insects and in agriculture and livestock. Flumethrin is a pyrethroid that is used against ectoparasites in many animals. The goal of this study was to evaluate the cytotoxic, apoptotic, genotoxic, and estrogenic effects of flumethrin on the mammalian breast cancer cell line (MCF-7). Compared with control groups, a dose-dependent decrease was observed in cell viability at concentrations of 100 µM and higher. The cytotoxic and apoptotic effects detected by LDH assay and AO/EtBr staining increased significantly at a concentration of 1000 µM. The expression of BCL2, which is an anti-apoptotic gene, significantly decreased, whereas BAX, TP53, and P21 expression significantly increased. The results of a comet assay indicated that flumethrin significantly changed tail length, tail % DNA, tail moment, and Olive tail moment in concentrations above 1 and 10 µM. In addition, a 0.1 µM concentration of flumethrin affected ERα receptor mediated cell proliferation and increased transcription of estrogen-responsive pS2 (TFF1) and progesterone receptor (PGR) genes. As a result, flumethrin-induced apoptosis and cytotoxicity at a high concentration, while induced genotoxicity even at lower concentrations. Flumethrin is an endocrine disrupting insecticide with estrogenic effects at very low concentrations. |
Databáze: | OpenAIRE |
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