Cellular uptake and apoptotic potential of rhenium labeled magnetic protein cages in MDA-MB-231 cells
| Autor: | Mürvet Volkan, Elif Asik, N. Tülin Güray, Tuğba Nur Aslan |
|---|---|
| Přispěvatelé: | OpenMETU |
| Rok vydání: | 2018 |
| Předmět: |
Cell Survival
Health Toxicology and Mutagenesis Iron Survivin Breast Neoplasms 02 engineering and technology 010402 general chemistry Toxicology 01 natural sciences chemistry.chemical_compound Annexin Cell Line Tumor Proto-Oncogene Proteins medicine Humans Propidium iodide Cytotoxicity Magnetite Nanoparticles bcl-2-Associated X Protein Pharmacology Radioisotopes Chemistry Cancer Oxides General Medicine 021001 nanoscience & nanotechnology medicine.disease Molecular biology Endocytosis 0104 chemical sciences Gene Expression Regulation Neoplastic Rhenium Proto-Oncogene Proteins c-bcl-2 Cell culture Apoptosis Cancer cell Apoferritins Female Drug Screening Assays Antitumor 0210 nano-technology Apoptosis Regulatory Proteins |
| Zdroj: | Environmental toxicology and pharmacology. 63 |
| ISSN: | 1872-7077 |
| Popis: | 188 Re-magnetoferritin nanoparticles (NPs) provide an attractive platform for localized radiation therapy due to their magnetic targeting capability while enhancing contrast in magnetic resonans imaging (MRI) signals. In this study, cellular uptake , in vitro cytotoxicity, apoptotic potential of a non-radioactive isotope of rhenium in the form of 187 Re-magnetoferritin NPs were evaluated in both human normal mammary epithelial and breast metastatic adenocarcinoma cell lines. The results showed that, NP administration into the cells is through receptor mediated endocytosis and cancer cells displayed significantly higher uptake and cytotoxicity compared to normal cells. IC 50 values of nanoparticles were calculated as 0.96 mg/mL for cancer and 1.73 mg/mL for normal cells. Annexin V/ Propidium Iodide (PI) staining also showed that, NPs induced higher apoptotic rates in cancer cells compared to normal cells. Gene expression analyses confirming the results showed that, pro-apoptotic PUMA and BAX genes were significantly up-regulated while anti-apoptotic BCL-2 and SURVIVIN genes were down-regulated in cancer cells compared to normal cells. Overall, these in vitro results suggest that, 187 Re-magnetoferritin NPs have a promising potential for cancer therapy and can be used for imaging and diagnostic purposes for breast cancer at concentrations lower than 0.96 mg/mL. At concentrations above 1 mg/mL, NPs induce apoptosis which can also be used for cancer treatments. |
| Databáze: | OpenAIRE |
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