Label-free single-cell protein quantification using a drop-based mix-and-read system
| Autor: | Ying Zhou, Yukun Ren, Haruichi Asahara, David A. Weitz, Ye Tao, Lloyd W. Ung, Shaorong Chong, John A. Heyman, Dongxian Yue, Alireza Abbaspourrad, Stephan A. Koehler, Roy Ziblat, Naiwen Cui, Huidan Zhang |
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| Rok vydání: | 2015 |
| Předmět: |
0303 health sciences
Multidisciplinary Chromatography Chemistry Drop (liquid) Cell 010402 general chemistry Mass spectrometry 01 natural sciences Antibody labeling Molecular biology Article 0104 chemical sciences 03 medical and health sciences medicine.anatomical_structure medicine Single-cell protein 030304 developmental biology Label free |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Quantitative protein analysis of single cells is rarely achieved due to technical difficulties of detecting minute amounts of proteins present in one cell. We develop a mix-and-read assay for drop-based label-free protein analysis of single cells. This high-throughput method quantifies absolute, rather than relative, amounts of proteins and does not involve antibody labeling or mass spectrometry. |
| Databáze: | OpenAIRE |
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