Anti-inflammatory and Antioxidant Effects ofLotus corniculatuson Paracetamol-induced Hepatitis in Rats
| Autor: | Amedah Souad, Samir Benayache, Abderahmane Mezrag, Fadila Benayache, Mohamed Bouheroum, Nacera Baali |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
antioxidant Antioxidant paracetamol medicine.medical_treatment Immunology Anti-Inflammatory Agents Pharmacology medicine.disease_cause Article Antioxidants Hepatitis Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lotus corniculatus Picrates Lactate dehydrogenase medicine Animals Humans Immunology and Allergy Rats Wistar Cells Cultured Acetaminophen 030304 developmental biology 0303 health sciences biology Plant Extracts Anti-inflammatory effect Biphenyl Compounds General Medicine Glutathione biology.organism_classification Malondialdehyde Rats myeloperoxidase Oxidative Stress Liver chemistry 030220 oncology & carcinogenesis Myeloperoxidase Lotus biology.protein Oxidative stress |
| Zdroj: | Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry |
| ISSN: | 1871-5230 |
| DOI: | 10.2174/1871523018666190222120752 |
| Popis: | Background:Herbal medicines have been used in the treatment of liver diseases for a long time. The current study was elaborated to evaluate in vitro and in vivo antioxidant and anti-inflammatory effects of Lotus corniculatus (L. corniculatus) butanolic extract.Methods:The in vitro antioxidant and anti-inflammatory properties of L. corniculatus were investigated by employing DPPH radical scavenging, H2O2 scavenging and BSA denaturation assays. In vivo antioxidant and anti-inflammatory effects of L. corniculatus were evaluated against paracetamol (APAP)-induced hepatitis in rats. L.corniculatus at doses of 100 and 200 mg/kg was administered orally once daily for seven days. Serum transaminases (AST and ALT) and lactate dehydrogenase (LDH), total bilirubin levels, liver malondialdehyde (MDA), reduced glutathione (GSH), glutathione S- transferase (GST) and superoxide dismutase (SOD) levels and inflammatory markers, such as serum Creactive protein (CRP), circulating and liver myeloperoxidase (MPO) levels were investigated. Further histopathological analysis of the liver sections was performed to support the effectiveness of L. corniculatus.Results:L. corniculatus exhibited strong antioxidant and anti-inflammatory effects in vitro. In the in vivo study, our findings demonstrate that L. corniculatus (100 and 200 mg/kg) administration led to an amelioration of APAP effects on liver histology, liver functions parameters (AST, ALT, LDH, and total bilirubin levels) and liver oxidative stress markers (MDA, GSH, GST and SOD levels). Furthermore, serum CRP, circulating MPO and liver MPO levels were declined by both doses of L. corniculatus extract. The best benefits were observed with 200 mg/kg of L. corniculatus extract.Conclusion:Antioxidant and anti-inflammatory effects of L. corniculatus extract may be due to the presence of active components. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|