Cholic Acid Attenuates ER Stress-Induced Cell Death in Coxsackievirus-B3 Infection
| Autor: | Hae In Jeong, Jisoo Yoon, Cheol-woo Park, Jae-young Han, Sang-Jip Nam, Jaeyoung Ko, Byung-Kwan Lim |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Programmed cell death viruses Blotting Western Cholic Acid Coxsackievirus Real-Time Polymerase Chain Reaction Virus Replication Antiviral Agents Applied Microbiology and Biotechnology HeLa 03 medical and health sciences chemistry.chemical_compound Western blot medicine Humans Messenger RNA Cell Death biology medicine.diagnostic_test Chemistry Gene Expression Profiling Cholic acid virus diseases RNA General Medicine Endoplasmic Reticulum Stress biology.organism_classification Molecular biology Enterovirus B Human 030104 developmental biology Unfolded protein response HeLa Cells Biotechnology |
| Zdroj: | Journal of Microbiology and Biotechnology. 28:109-114 |
| ISSN: | 1738-8872 1017-7825 |
| DOI: | 10.4014/jmb.1708.08009 |
| Popis: | Coxsackievirus Type B3 (CVB3) is an enterovirus that belongs to the Picornaviridae and causes various diseases such as myocarditis and hand-foot-mouth disease. However, an effective antiviral drug is still not developed. In this study, we looked for potential inhibitors of CVB3 replication by examining the survival of CVB3-infected HeLa cells. We detected an antiviral effect by cholic acid and identified it as a candidate inhibitor of CVB3 replication. Cholic acid circulates in the liver and intestines, and it helps the digestion and absorption of lipids in the small intestine. HeLa cells were cultured in 12-well plates and treated with cholic acid (1 and 10 μg/ml) and 10⁶ PFU/ml of CVB3. After 16 h post-infection, the cells were lysed and subjected to western blot analysis and RT-PCR. The production of the viral capsid protein VP1 was dramatically decreased, and translation initiation factor eIF4G1 cleavage was significantly inhibited by treatment with 10 μg/ml cholic acid. Moreover, cholic acid inhibited ERK signaling in CVB3-infected HeLa cells. RT-PCR showed that the amounts of the CVB3 RNA genome and mRNA for the ER stress-related transcription factor ATF4 were significantly reduced. These results showed that cholic acid strongly reduced ER stress and CVB3 proliferation. This compound can be developed as a safe natural therapeutic agent for enterovirus infections. |
| Databáze: | OpenAIRE |
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