Isolation and Culturing Primary Cholangiocytes from Mouse Liver
| Autor: | Mary P. Mullen, Alexander Miethke, Gregory M. Tiao, Bal Krishan Sharma, Bryan Donnelly, Ramesh Kudira, Sujit K. Mohanty |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Liver injury
Pathology medicine.medical_specialty Strategy and Management Mechanical Engineering Gallbladder Metals and Alloys Biology medicine.disease Bone canaliculus digestive system Industrial and Manufacturing Engineering Cholangiocyte Primary sclerosing cholangitis medicine.anatomical_structure Cholestasis Fibrosis Biliary atresia medicine Methods Article |
| Zdroj: | Bio Protoc |
| Popis: | Cholangiocytes are epithelial cells lining the intrahepatic and extrahepatic bile ducts. Cholangiocytes perform key physiological functions in the liver. Bile synthesized by hepatocytes is secreted into bile canaliculi, further stored in the gallbladder, and finally discharged into the duodenum. Due to liver injury, biliary epithelial proliferate in response to endogenous or exogenous signals leading to cholangiopathies, inflammation, fibrosis, and cholangiocarcinoma. Cholangiocytes exhibit anatomical and functional heterogeneity, and understanding such diversified functions will potentially help in finding effective therapies for various cholestatic liver diseases. To perform such functional studies, effective cholangiocyte isolation and culture procedures are needed. This protocol will aid in easy isolation and expansion of cholangiocytes from the liver. |
| Databáze: | OpenAIRE |
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