Angiotensin type 2 receptor activation limits kidney injury during the early phase and induces Treg cells during the late phase of renal ischemia
| Autor: | Tahir Hussain, Sanket Patel, Riyasat Ali |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Agonist
medicine.medical_specialty Time Factors Physiology medicine.drug_class Anti-Inflammatory Agents Thiophenes Kidney Receptor Angiotensin Type 2 T-Lymphocytes Regulatory Rats Sprague-Dawley Late phase Internal medicine medicine Humans Animals Sulfonamides Renal ischemia business.industry FOXP3 Acute Kidney Injury Chemotaxis Leukocyte Disease Models Animal Interleukin 10 Phenotype Editorial medicine.anatomical_structure Endocrinology Reperfusion Injury Cytokines Kidney Diseases Angiotensin II Type-2 Receptor Early phase business Research Article Signal Transduction |
| Zdroj: | Am J Physiol Renal Physiol |
| ISSN: | 1522-1466 1931-857X |
| DOI: | 10.1152/ajprenal.00507.2020 |
| Popis: | Kidney infiltrating immune cells such as monocytes, neutrophils, and T cells play critical roles in renal ischemia-reperfusion (IR) injury and repair. Recently, the angiotensin II type 2 receptor (AT(2)R) has been implicated in protecting kidneys against injury and monocyte infiltration, particularly in chronic kidney disease. However, the role of AT(2)R in IR injury and repair phases and T cell modulation is unknown. To address this question, Sprague–Dawley rats were subjected to IR with or without AT(2)R agonist C21 treatment. IR caused early (2 h postreperfusion) renal functional injury (proteinuria, plasma urea, and creatinine) and enhanced immune cells (T cells and CD4 T cells) infiltration and levels of the proinflammatory cytokines monocyte chemoattractant protein-1, TNF-α, and IL-6. C21 treatment reversed these changes but increased the anti-inflammatory IL-10 level. On day 3, C21 treatment increased CD4(+)FoxP3(+) (regulatory T cells) and CD4(+)IL-10(+) cells and reduced kidney injury molecule-1 and neutrophil gelatinase-associated lipocalin in the kidney compared with the IR control, suggesting the involvement of AT(2)R in kidney repair. These data indicate that AT(2)R activation protects the kidney against IR injury and immune cell infiltration in the early phase and modulates CD4 T cells toward the regulatory T cell phenotype, which may have long-term beneficial effects on kidney function. NEW & NOTEWORTHY The angiotensin II type 2 receptor agonist C21 has been known to have a renoprotective role in various kidney pathologies. C21 treatment (before renal ischemia) attenuated postischemic kidney injury, kidney dysfunction, and immune cell infiltration during the injury phase. Also, C21 treatment modulated the kidney microenvironment by enhancing anti-inflammatory responses mainly mediated by IL-10. During the repair phase, C21 treatment enhanced IL-10-secreting CD4 T cells and FoxP3-secreting regulatory T cells in Sprague–Dawley rats. |
| Databáze: | OpenAIRE |
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