Molecular identification of Neoechinorhynchus rutili parasite diagnosed in some fish species caught in Menzelet dam lake in Kahramanmaras province (Turkey)
| Autor: | Mikail Özcan, Neslihan Bozdoğan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
biology
Fish species Zoology biology.organism_classification DNA extraction Article Cyprinus law.invention Parasite Neoechinorhynchus rutili Fish PCR lcsh:Biology (General) law Fish Parasite hosting General Agricultural and Biological Sciences lcsh:QH301-705.5 Polymerase chain reaction Molecular identification |
| Zdroj: | Saudi Journal of Biological Sciences, Vol 27, Iss 7, Pp 1717-1721 (2020) Saudi Journal of Biological Sciences |
| Popis: | This study details the molecular identification of the parasite Neoechinorhynchus rutili diagnosed in fish (Capoeta barroisi, Cyprinus carpio, Barbus rajanorum) caught in Menzelet Dam Lake in Kahramanmaras Province, Turkey. Parasite samples were obtained from the intestines of fish caught from January to June of 2013. The collected parasites were stored in sample vials containing 70% alcohol. Using staining methods and based on morphology, 120 N. rutili specimens were identified. DNA isolation of N. rutili was accomplished using special tissue sets for parasites. Specific primers were utilized in the molecular identification of N. rutili using polymerase chain reaction (PCR) and it was possible to verify that all of the parasites contained N. rutili molecules. In conclusion, using multiple methods we successfully identified and confirmed the presence of N. rutili parasites in the fish caught in Kahramanmaras Province. The process of identification of N. rutili using morphology and staining methods is time-consuming; however, PCR was successfully performed in a short time to accomplish the same results. The success of this study may lead to more original and extensive work aimed at the efficient molecular identification of parasitic agents found in fish. |
| Databáze: | OpenAIRE |
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