Enhanced direct electron transfer between laccase and hierarchical carbon microfibers/carbon nanotubes composite electrodes. Comparison of three enzyme immobilization methods
| Autor: | Leonard Stoica, Yvonne Beyl, Wenzhi Jia, Wolfgang Schuhmann, Cristina Gutierrez-Sanchez, Marcos Pita, Antonio L. De Lacey |
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| Přispěvatelé: | European Commission |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Immobilized enzyme
General Chemical Engineering Carboxylic acid Inorganic chemistry Carbon nanotubes chemistry.chemical_element 02 engineering and technology Carbon nanotube Hierarchical carbon composite Trametes hirsuta 010402 general chemistry 01 natural sciences law.invention Oxygen reduction reaction Electron transfer law Electrochemistry Graphite Laccase chemistry.chemical_classification biology Chemistry 021001 nanoscience & nanotechnology biology.organism_classification Biofuel cells 0104 chemical sciences Direct electron transfer 0210 nano-technology Carbon Biocathode |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 18 páginas, 4 figuras Three immobilization protocols were investigated with respect to direct electron transfer between hierarchical carbon microfibers/carbon nanotubes composite material on graphite rod electrodes and Trametes hirsuta laccase. Immobilization was done by covalent binding of laccase to aminophenyl-modified electrodes via amide-bond formation with carboxylic acid residues or imino-bond formation with aldehyde groups introduced by oxidation of sugar residues of the enzyme's glycosylation shell. Moreover, immobilization was achieved by adsorbing laccase to electrodes hydrophilized with pyrene-hexanoic acid. High current densities for biocatalytic oxygen reduction were obtained for all immobilization strategies. The formation of the imino bonds let to the binding of laccase in close to 100% direct electron transfer configuration and consequently to the highest oxygen reduction currents. The authors are grateful to the EU for financial support in the framework of the project “3D-Nanobiodevice” (NMP4-SL-2009-229255). |
| Databáze: | OpenAIRE |
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