Effect of three commercial extenders on sperm motility and fertility in liquid ram semen stored at 15 °C or 5 °C
| Autor: | Carlos C. Pérez-Marín, José M. León, María Gabriela Pizarro, S. Nogales, A. Arando, Francisco Javier Navas-Gonzalez, Juan Vicente Delgado |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
food.ingredient
Semen Diluent law.invention 03 medical and health sciences 0302 clinical medicine food Animal science law Yolk Skimmed milk Animals Sheep Domestic Sperm motility Cryopreservation 030219 obstetrics & reproductive medicine General Veterinary Chemistry Extender Temperature 0402 animal and dairy science food and beverages 04 agricultural and veterinary sciences 040201 dairy & animal science Sperm Dilution Semen Analysis Fertility Sperm Motility Semen Preservation |
| Zdroj: | Acta Veterinaria Hungarica. 67:430-444 |
| ISSN: | 1588-2705 0236-6290 |
| DOI: | 10.1556/004.2019.043 |
| Popis: | The effect of different extenders on sperm motility and fertility was evaluated during liquid storage of ram semen at 5 °C and 15 °C. The semen was collected, pooled and diluted in three commercial extenders: Inra 96® (INRA) based on skimmed milk, Biladyl® A fraction (BIL) based on egg yolk, and Ovixcell® (OVIX) based on soybean lecithin. Then, sperm motility was evaluated at 0, 6, 24, 48, 72 and 96 h. In order to evaluate fertility, samples stored at 15 °C were used after dilution in INRA and OVIX. Results showed that progressive motility was significantly higher up to 72 h of storage in sperm samples maintained at 5 °C in comparison with 15 °C, similarly for each tested diluent. When samples were stored at 5 °C in OVIX, kinematic parameters such as velocity (except curvilinear velocity, VCL), trajectory [linearity (LIN), straightness (STR), wobble (WOB)], amplitude of lateral head displacement (ALH) and beat/cross frequency (BCF) were higher than in INRA and BIL. No significant differences in pregnancy rate were detected between INRA (62.6%) and OVIX (58.9%). In conclusion, liquid storage at 5 °C with OVIX extender is an interesting option since non-animal components are used, and this extender offers similar in vitro and in vivo efficacy as other extenders containing animal components. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|