Roles of 5-HT on phase transition of neurite outgrowth in the identified serotoninergic neuron C1, Helisoma trivolvis
| Autor: | Hyeon-Cheol Cha, Won-Cheoul Jang, Glen B. Baker, Kee-Chan Ahn, Mee-Sook Song, Myung Jin Moon |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell signaling Serotonin Time Factors Neurite Neuronal Outgrowth Snails Motility Tryptophan Hydroxylase 03 medical and health sciences Cellular and Molecular Neuroscience Developmental Neuroscience medicine Animals Biogenic Monoamines Growth cone Cells Cultured Chromatography High Pressure Liquid Helisoma Cerebral Cortex biology Dose-Response Relationship Drug Fenclonine biology.organism_classification Neuroregeneration Cell biology 030104 developmental biology medicine.anatomical_structure Neuron Serotonin Antagonists Filopodia Serotonergic Neurons |
| Zdroj: | Invertebrate neuroscience : IN. 18(3) |
| ISSN: | 1439-1104 |
| Popis: | Neurite outgrowth is a morphological marker of neuronal differentiation and neuroregeneration, and the process includes four essential phases, namely initiation, elongation, guidance and cessation. Intrinsic and extrinsic signaling molecules seem to involve morphological changes of neurite outgrowth via various cellular signaling cascades phase transition. Although mechanisms associated with neurite outgrowth have been studied extensively, little is known about how phase transition is regulated during neurite outgrowth. 5-HT has long been studied with regard to its relationship to neurite outgrowth in invertebrate and vertebrate culture systems, and many studies have suggested 5-HT inhibits neurite elongation and growth cone motility, in particular, at the growing parts of neurite such as growth cones and filopodia. However, the underlying mechanisms need to be investigated. In this study, we investigated roles of 5-HT on neurite outgrowth using single serotonergic neurons C1 isolated from Helisoma trivolvis. We observed that 5-HT delayed phase transitions from initiation to elongation of neurite outgrowth. This study for the first time demonstrated that 5-HT has a critical role in phase-controlling mechanisms of neurite outgrowth in neuronal cell cultures. |
| Databáze: | OpenAIRE |
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