Effects of Choto-san and hooks and stems of Uncaria sinensis on antioxidant enzyme activities in the gerbil brain after transient forebrain ischemia
| Autor: | Hisao Nishijo, Takako Yokozawa, Kazufumi Kouta, Yutaka Shimada, Etsuro Hori, Koichi Yokoyama, Takako Nakagawa, Shinobu Takagi, Katsutoshi Terasawa, Nobuyasu Sekiya |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Hippocampus Hippocampal formation Pharmacology Biology Gerbil Antioxidants Brain Ischemia Brain ischemia Superoxide dismutase chemistry.chemical_compound Prosencephalon parasitic diseases Drug Discovery medicine Animals chemistry.chemical_classification Plant Stems Superoxide Glutathione peroxidase Anatomy medicine.disease biology.organism_classification Uncaria chemistry biology.protein Gerbillinae Drugs Chinese Herbal |
| Zdroj: | Journal of Ethnopharmacology. 95:335-343 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2004.08.018 |
| Popis: | Previously, we revealed that oral administrations of Choto-san, a Kampo formula, and the hooks and stems of Uncaria sinensis Haviland (Rubiaceae), a medicinal plant comprising Choto-san, enhanced superoxide anion and hydroxyl radical scavenging activities in the hippocampus, and prevented delayed neuronal death of pyramidal cells in the hippocampal CA1 region in a transient forebrain ischemia gerbil model. In the present study, for the purpose of clarifying whether the endogenous antioxidant enzymes contribute to these mechanisms, we investigated the effects of Choto-san extract (CSE) and Uncaria sinensis extract (USE) on superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities in the brain by using the same experimental model. 1.0% CSE or 3.0% USE were dissolved in water and provided to gerbils ad libitum from 7 days prior to ischemia/reperfusion (i/rp). Seven days of continuous administrations of CSE or USE without i/rp procedure enhanced CAT activity but not SOD and GSH-Px activities in both the hippocampus and cortex. CSE elevated CAT activity in the hippocampus at 7 days and in the cortex at 3h after i/rp. USE raised CAT activity in both the hippocampus and cortex at 3 h and 7 days after i/rp. These results suggest that one of the mechanisms of the protective effects of CSE and USE against transient brain ischemia-induced neuronal damage may be their enhancing effect on CAT activity in the brain. |
| Databáze: | OpenAIRE |
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