Correlation between endoglin and malignant phenotype in human melanoma cells: Analysis of hsa-mir-214 and hsa-mir-370 in cells and their extracellular vesicles
| Autor: | Lidia Ruiz-Llorente, María Jesús Ruiz-Rodríguez, Claudia Savini, Teresa González-Muñoz, Erica Riveiro-Falkenbach, José L. Rodríguez-Peralto, Héctor Peinado, Carmelo Bernabeu |
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| Přispěvatelé: | Ministerio de Ciencia, Innovación y Universidades (España), Consejo Superior de Investigaciones Científicas (España), Fondo de Investigación Sanitaria (FIS) de la Seguridad Social, Centro de Investigación Biomédica en Red Enfermedades Raras (España), Instituto de Salud Carlos III, European Coal and Steel Community, Ruiz-Llorente, Lidia, González-Muñoz, Teresa, Rodriguez-Peralto, José L., Peinado, Héctor, Bernabéu, Carmelo |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Advances in Experimental Medicine and Biology ISBN: 9783031261626 |
| Popis: | 20 p.-8 fig.-1 tab. Endoglin (CD105) is an auxiliary receptor of transforming growth factor (TGF)-β family members that is expressed in human melanomas. It is heterogeneously expressed by primary and metastatic melanoma cells, and endoglin targeting as a therapeutic strategy for melanoma tumors is currently been explored. However, its involvement in tumor development and malignancy is not fully understood. Here, we find that endoglin expression correlates with malignancy of primary melanomas and cultured melanoma cell lines. Next, we have analyzed the effect of ectopic endoglin expression on two miRNAs (hsa-mir-214 and hsa-mir-370), both involved in melanoma tumor progression and endoglin regulation. We show that compared with control cells, overexpression of endoglin in the WM-164 melanoma cell line induces; (i) a significant increase of hsa-mir-214 levels in small extracellular vesicles (EVs) as well as an increased trend in cells; and (ii) significantly lower levels of hsa-mir-370 in the EVs fractions, whereas no significant differences were found in cells. As hsa-mir-214 and hsa-mir-370 are not just involved in melanoma tumor progression, but they can also target endoglin-expressing endothelial cells in the tumor vasculature, these results suggest a complex and differential regulatory mechanism involving the intracellular and extracellular signaling of hsa-mir-214 and hsa-mir-370 in melanoma development and progression. This work was supported by grants from Ministerio de Ciencia, Innovación y Universidades (SAF2013-43421-R to CB), Consejo Superior de Investigaciones Científicas (201920E022 to CB), Fondo de Investigación Sanitaria (FIS) de la Seguridad Social (PI-20/01553 to JLR-P), and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER;ISCIII-CB06/07/0038 to CB and contract CNV-234-PRF-360 to LR-L) of Spain. CIBERER is an initiative of the Instituto de Salud Carlos III (ISCIII) of Spain supported by FEDER funds. The CNIO, certified as a Severo Ochoa Excellence Centre, is supported by the Spanish government through the ISCIII. |
| Databáze: | OpenAIRE |
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