Curing cytoplasmic male sterility via TALEN-mediated mitochondrial genome editing
| Autor: | Nobuya Koizuka, Miki Okuno, Shungo Yanase, Shin-ichi Arimura, Chie Koizuka, Yu Tsuruta, Hajime Sugaya, Nobuhiro Tsutsumi, Tomohiko Kazama, Yuta Watari, Atsushi Toyoda, Takehiko Itoh, Kinya Toriyama |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Mitochondrial DNA Nuclear gene Plant Infertility Sterility Plant Science Biology 01 natural sciences 03 medical and health sciences Gene Knockout Techniques Transcription Activator-Like Effector Nucleases Homologous Recombination Gene Genetics Gene Editing Transcription activator-like effector nuclease Cytoplasmic male sterility Brassica napus food and beverages Oryza Mitochondria 030104 developmental biology Genome Mitochondrial Mitochondrion localization Homologous recombination 010606 plant biology & botany |
| Zdroj: | Nature plants. 5(7) |
| ISSN: | 2055-0278 |
| Popis: | Sequence-specific nucleases are commonly used to modify the nuclear genome of plants. However, targeted modification of the mitochondrial genome of land plants has not yet been achieved. In plants, a type of male sterility called cytoplasmic male sterility (CMS) has been attributed to certain mitochondrial genes, but none of these genes has been validated by direct mitochondrial gene-targeted modification. Here, we knocked out CMS-associated genes (orf79 and orf125) of CMS varieties of rice and rapeseed, respectively, using transcription activator-like effector nucleases (TALENs) with mitochondria localization signals (mitoTALENs). We demonstrate that knocking out these genes cures male sterility, strongly suggesting that these genes are causes of CMS. Sequencing revealed that double-strand breaks induced by mitoTALENs were repaired by homologous recombination, and that during this process, the target genes and surrounding sequences were deleted. Our results show that mitoTALENs can be used to stably and heritably modify the mitochondrial genome in plants. |
| Databáze: | OpenAIRE |
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