Nuclear matrix protein expressions in hepatocytes of normal and cirrhotic rat livers under normal and regenerating conditions
| Autor: | Choong Tsek Liew, Mei Lin Jin, H. K Richard Li, Han Liang Lin, Paul B.S. Lai, Eng Ching Chew, Ming Xiao Ding, Jing Ping Yun, Wan Yee Lau, Yam Hin Fai, Xiao Yu Yin, Ming Tao Li |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
Cirrhosis medicine.medical_treatment Vimentin Thioacetamide Liver Cirrhosis Experimental Biochemistry Cytokeratin Nuclear Matrix-Associated Proteins Western blot medicine Animals Rats Wistar Molecular Biology biology medicine.diagnostic_test Gene Expression Profiling Cell Biology Nuclear matrix medicine.disease Molecular biology Liver regeneration Liver Regeneration Rats Hsp70 Gene Expression Regulation Immunology Hepatocytes biology.protein Hepatectomy |
| Zdroj: | Journal of Cellular Biochemistry. 91:1269-1279 |
| ISSN: | 1097-4644 0730-2312 |
| DOI: | 10.1002/jcb.20034 |
| Popis: | We explored the feasibility of studying nuclear matrix protein (NMP) expressions of the hepatocytes in normal and cirrhotic rat livers with liver regeneration after partial hepatectomy. Sixteen Wistar healthy rats were studied with experimental liver regeneration and/or liver cirrhosis. Two-dimensional (2-D) gel electrophoresis was used to generate these NMP compositions from these rat liver samples. Several antibodies against cytokeratin, vimentin, actin, B23, HNF4alpha, and heat shock protein 70 were used for identification by Western blot. Totally, 41 strongly stained protein spots were characterized on the 2-D gels. Thirty-four protein spots were detected in all of these rat livers, of which, cytokeratin, vimentin, actin, HNF4alpha, and heat shock protein 70 were identified. B23 was detected in the regenerated livers. Three protein spots (s33, s34, and s35) were detectable only in NMP preparation extracted from the regenerating rat livers after hepatectomy. Another three protein spots (s36, s37, and s38) were detectable only in NMP preparation extracted from thioacetamide-induced cirrhotic rat livers. Under these conditions including experimental liver regeneration and/or liver cirrhosis, Over thirty higher abundance NMPs of hepatocytes were consistently expressed and considered as common and basic NMPs. Some of the NMPs are specific for liver regeneration and may play a critical role in cell proliferation and cell cycle, and some are specific for liver cirrhosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text