Pseudomonas aeruginosa ATCC 9027 is a non-virulent strain suitable for mono-rhamnolipids production
| Autor: | Abigail González-Valdez, Gabriel Yaxal Ponce-Soto, Luis Servín-González, Rosario Morales-Espinosa, José-Luis Méndez, María-Victoria Grosso-Becerra, Gloria Soberón-Chávez, María-Jessica Granados-Martínez, Gabriela Delgado, Miguel Cocotl-Yañez, Estefanía Morales |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Operon 030106 microbiology Virulence Heterologous Biology medicine.disease_cause Rhamnose Applied Microbiology and Biotechnology Microbiology Metabolic engineering Mice Surface-Active Agents 03 medical and health sciences Plasmid Drug Resistance Bacterial medicine Animals Pseudomonas Infections Gene Strain (chemistry) Pseudomonas aeruginosa Decanoates Sequence Analysis DNA General Medicine Disease Models Animal 030104 developmental biology Metabolic Engineering Genome Bacterial Metabolic Networks and Pathways Plasmids Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 100:9995-10004 |
| ISSN: | 1432-0614 0175-7598 |
| Popis: | Rhamnolipids produced by Pseudomonas aeruginosa are biosurfactants with a high biotechnological potential, but their extensive commercialization is limited by the potential virulence of P. aeruginosa and by restrictions in producing these surfactants in heterologous hosts. In this work, we report the characterization of P. aeruginosa strain ATCC 9027 in terms of its genome-sequence, virulence, antibiotic resistance, and its ability to produce mono-rhamnolipids when carrying plasmids with different cloned genes from the type strain PAO1. The genes that were expressed from the plasmids are those coding for enzymes involved in the synthesis of this biosurfactant (rhlA and rhlB), as well as the gene that codes for the RhlR transcriptional regulator. We confirm that strain ATCC 9027 forms part of the PA7 clade, but contrary to strain PA7, it is sensitive to antibiotics and is completely avirulent in a mouse model. We also report that strain ATCC 9027 mono-rhamnolipid synthesis is limited by the expression of the rhlAB-R operon. Thus, this strain carrying the rhlAB-R operon produces similar rhamnolipids levels as PAO1 strain. We determined that strain ATCC 9027 with rhlAB-R operon was not virulent to mice. These results show that strain ATCC 9027, expressing PAO1 rhlAB-R operon, has a high biotechnological potential for industrial mono-rhamnolipid production. |
| Databáze: | OpenAIRE |
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