High-fat diet effect on periapical lesions and hepatic enzymatic antioxidant in rats
| Autor: | Pauline Mastella Lang, Maria M. Campos, Raquel Cristine Silva Barcelos, Carlos Alexandre Souza Bier, Natália Brezolin Zago, Carlos Frederico Brilhante Wolle, Adriana Etges, Camilla dos Santos Tibúrcio-Machado, Carlos Eduardo Leite |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Antioxidant medicine.medical_treatment Blood lipids Diet High-Fat medicine.disease_cause 030226 pharmacology & pharmacy Antioxidants General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals Rats Wistar General Pharmacology Toxicology and Pharmaceutics Inflammation Periodontitis Glucose tolerance test Periapical periodontitis medicine.diagnostic_test biology business.industry General Medicine Glutathione Glucose Tolerance Test Catalase medicine.disease Lipids Rats Oxidative Stress 030104 developmental biology Endocrinology Liver chemistry biology.protein Insulin Resistance business Oxidative stress |
| Zdroj: | Life Sciences. 264:118637 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/j.lfs.2020.118637 |
| Popis: | To evaluate the effects of a high-fat diet (HFD) on the progression of apical periodontitis (AP), local inflammation, systemic antioxidant status, and blood lipid profile in rats.Sixteen male Wistar rats were fed a standard diet (SD) or a HFD. At the sixth experimental week, the pulp chambers of the mandibular first molars were exposed to develop AP. A glucose tolerance test was performed the week before euthanasia. At the tenth experimental week, the animals were euthanized and the livers were collected to estimate catalase (CAT) and reduced glutathione (GSH) levels. Blood was acquired for biochemical analysis. The size of AP was estimated from radiographs and described as AP size-to-body weight ratio; inflammatory grade of AP was determined by histological analysis.At the end of the experimental period, the rats fed the HFD had 30% less weight (P 0.0001) and higher blood glucose levels after 30 min of sucrose intake (P 0.05) than those fed the SD. Animals from the HFD group had lower levels of CAT (P 0.01), but the same was not observed in the GSH levels. Plasma insulin and total cholesterol were not affected by the diet. The rats fed the HFD presented greater AP than those fed the SD (P 0.05). However, the local inflammatory infiltrate was similar in both groups.The alterations promoted by the consumption of a HFD were not only observed systemically, but also locally, producing greater AP in rats than a SD. |
| Databáze: | OpenAIRE |
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