Nitric oxide and macrophage antiviral extrinsic activity
| Autor: | M C Courreges, F Benencia |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_treatment
viruses Herpesvirus 2 Human Intraperitoneal injection Immunology Dose dependence Herpesvirus 1 Human Pharmacology Nitric Oxide Virus Replication Nitric oxide Keratitis Pathogenesis chemistry.chemical_compound Mice Chlorocebus aethiops medicine Macrophage Animals Immunology and Allergy Nitrite Vero Cells Nitrites Mice Inbred BALB C omega-N-Methylarginine Dose-Response Relationship Drug Macrophage Activation medicine.disease chemistry Vero cell Keratitis Herpetic Macrophages Peritoneal Female Original Article |
| Zdroj: | Immunology |
| ISSN: | 1365-2567 0019-2805 |
| DOI: | 10.1046/j.1365-2567.1999.00864.x |
| Popis: | In this study we evaluated the relationship between nitric oxide (NO) and macrophage antiviral extrinsic activity. Macrophages activated by intraperitoneal injection of herpes simplex virus-2 (HSV-2), showed both extrinsic antiviral activity and high nitrite production in contrast to non-activated, resident macrophages. The extrinsic antiviral activity was observed in cultures of Vero cells infected with HSV-1 and HSV-2. The NO inhibitor N-monomethyl-l-arginine acetate (l-NMA) impaired the antiviral activity of HSV-elicited macrophages. The effect was dose dependent and correlated with a reduction of nitrite in the culture media. The effect of l-NMA was reversed by the addition of l-arginine. These data indicate that NO could be responsible for the described activity. Furthermore, l-NMA treatment resulted in the aggravation of HSV-1-induced keratitis in the mouse model, supporting a defensive role of NO in the pathogenesis of HSV-1 corneal infection. |
| Databáze: | OpenAIRE |
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