Expression levels of the hypothalamic AMPK gene determines the responsiveness of the rats to electroacupuncture-induced analgesia
| Autor: | Joohun Ha, Sun Kwang Kim, Byung-Il Min, Giseog Lee, Fu-Shi Quan, Insop Shim, Hyunsu Bae, Heera Yoon, Ji Hwan Lee, Hyunseong Kim, Boram Sun, Sung-Hwa Sohn |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
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medicine.medical_specialty Electroacupuncture medicine.medical_treatment Hypothalamus Gene Expression 5’-AMP-activated protein kinase AMP-Activated Protein Kinases Energy homeostasis Rats Sprague-Dawley AMP-activated protein kinase Internal medicine Gene expression medicine Adenovirus Animals RNA Messenger Protein kinase A Microinjection Pain Measurement biology business.industry Reverse Transcriptase Polymerase Chain Reaction AMPK Nonresponder Responder General Medicine Rats Endocrinology Complementary and alternative medicine biology.protein Analgesia business Research Article |
| Zdroj: | BMC Complementary and Alternative Medicine |
| ISSN: | 1472-6882 |
| Popis: | Background Although electroacupuncture (EA) relieves various types of pain, individual differences in the sensitivity to EA analgesia have been reported, causing experimental and clinical difficulties. Our functional genomic study using cDNA microarray identified that 5’-AMP-activated protein kinase (AMPK), a well-known factor in the regulation of energy homeostasis, is the most highly expressed gene in the hypothalamus of the rats that were sensitive to EA analgesia (“responder”), as compared to the rats that were insensitive to EA analgesia (“non-responder”). In this study, we investigated the causal relationship between the hypothalamic AMPK and the individual variation in EA analgesia. Methods Sprague-Dawley (SD) rats were divided into the responder and the non-responder groups, based on EA-induced analgesic effects in the tail flick latency (TFL) test, which measures the latency of the tail flick response elicited by radiant heat applied to the tail. Real-time reverse transcription-polymerase chain reaction (RT-PCR) was performed to quantify the expression levels of AMPK mRNA in the hypothalamus of the responder and non-responder rats. Further, we examined whether viral manipulation of the AMPK expression in the hypothalamus modulates EA analgesia in rats. Results The real-time RT-PCR analysis showed that mRNA expression levels of AMPK in the hypothalamus of the responder rats are significantly higher than those of the non-responder rats, validating the previous microarray results. Microinjection of dominant negative (DN) AMPK adenovirus, which inhibits AMPK activity, into the rat hypothalamus significantly attenuates EA analgesia (p 0.05). Conclusions The present results demonstrated that levels of AMPK gene expression in the rat hypothalamus determine the individual differences in the sensitivity to EA analgesia. Thus, our findings provide a clinically useful evidence for the application of acupuncture or EA for analgesia. |
| Databáze: | OpenAIRE |
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