Systematic development and optimization of chemically defined medium supporting high cell density growth of Bacillus coagulans
| Autor: | Yu Chen, Yonghong Wang, Fengqing Dong |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
In silico 030106 microbiology Applied Microbiology and Biotechnology Models Biological 03 medical and health sciences chemistry.chemical_compound Metabolic flux analysis Computer Simulation biology Bacillus coagulans business.industry High cell General Medicine biology.organism_classification Metabolic Flux Analysis Flux balance analysis Lactic acid Biotechnology Culture Media Chemically defined medium 030104 developmental biology Metabolic Model chemistry Biological system business |
| Zdroj: | Applied microbiology and biotechnology. 100(18) |
| ISSN: | 1432-0614 |
| Popis: | With determined components and experimental reducibility, the chemically defined medium (CDM) and the minimal chemically defined medium (MCDM) are used in many metabolism and regulation studies. This research aimed to develop the chemically defined medium supporting high cell density growth of Bacillus coagulans, which is a promising producer of lactic acid and other bio-chemicals. In this study, a systematic methodology combining the experimental technique with flux balance analysis (FBA) was proposed to design and simplify a CDM. The single omission technique and single addition technique were employed to determine the essential and stimulatory compounds, before the optimization of their concentrations by the statistical method. In addition, to improve the growth rationally, in silico omission and addition were performed by FBA based on the construction of a medium-size metabolic model of B. coagulans 36D1. Thus, CDMs were developed to obtain considerable biomass production of at least five B. coagulans strains, in which two model strains B. coagulans 36D1 and ATCC 7050 were involved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|