Peptide-Based Photocathodic Biosensors: Integrating a Recognition Peptide with an Antifouling Peptide
| Autor: | Gao-Chao Fan, Di Zhang, Xiliang Luo, Xiao-Mei Shi, Shiting Gu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Protein molecules Biofouling Photochemistry Surface Properties Chemistry Photoelectron Spectroscopy 010401 analytical chemistry Peptide Nanotechnology Biointerface Biosensing Techniques Electrochemical Techniques 010402 general chemistry Sensitivity and Specificity 01 natural sciences 0104 chemical sciences Analytical Chemistry Immobilized Proteins Microscopy Electron Scanning Peptides Electrodes Biosensor Signal interference |
| Zdroj: | Analytical Chemistry. 93:2706-2712 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.0c05234 |
| Popis: | Accurate and sensitive detection of targets in practical biological matrixes such as blood, plasma, serum, or tissue fluid is a frontier issue for most biosensors since the coexistence of both potential reducing agents and protein molecules has the possibility of causing signal interference. Herein, aiming at detection in a complex environment, an advanced and robust peptide-based photocathodic biosensor, which integrated a recognition peptide with an antifouling peptide in one probe electrode, was first proposed. Selecting human chorionic gonadotropin (hCG) as a model target, the recognition peptide with the sequence PPLRINRHILTR was first anchored on the CuBi2O4/Au (CBO/Au) photocathode and then the antifouling peptide with the sequence EKEKEKEPPPPC was further anchored to generate an antifouling biointerface. The peptide-based photocathodic biosensor demonstrated excellent anti-interference to both nonspecific proteins and reducing agents because of the capability of the antifouling peptide. It also exhibited good sensitivity owing to the utilization of the recognition peptide rather than an antibody probe. This peptide-integrated method offers a new perspective for practical applications of photocathodic biosensors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|