Reversal of visceral hypersensitivity in rat by Menthacarin®, a proprietary combination of essential oils from peppermint and caraway, coincides with mycobiome modulation

Autor: E. Koch, Roy Christiaan Montijn, Theodorus B. M. Hakvoort, Jurgen Seppen, Evgeni Levin, Frank H. J. Schuren, W J de Jonge, R.M.J.G.J. van den Wijngaard, D. Maria-Ferreira, Sara Botschuijver, Olaf Welting
Přispěvatelé: AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, Graduate School, Gastroenterology and Hepatology, Tytgat Institute for Liver and Intestinal Research
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Unclassified drug
Visceral sensitivity
Physiology
Assay
Pharmacology
Digestive function
Treatment response
DNA 18S
Motor activity
Essential oil
law.invention
chemistry.chemical_compound
Species composition
0302 clinical medicine
law
Candida albicans
High throughput sequencing
Decanoic acid
Antifungal activity
Radial diffusion assay
Fluconazole
Irritable bowel syndrome
Priority journal
Visceral hypersensitivity
Intestine flora
Antibiotic agent
Gastroenterology
Mentha piperita
Antimicrobial
Gastrointestinal disorder
Digestive tract agent
Penicillin derivative
Streptomycin
Caraway
030211 gastroenterology & hepatology
Female
Antifungal agent
Drug mechanism
Bacillus subtilis
Physical sensitivity
Drug megadose
Abdominal pain
Biology
DNA 16S
Colorectal distension
Digestive system disease
Low drug dose
03 medical and health sciences
IBS
Octanoic acid
Dose response
medicine
Hypersensitivity
Animal model
Drug dose comparison
Microbiome
Animal experiment
Peppermint oil
Caraway oil
Menthacarin
Bacteria
Endocrine and Autonomic Systems
Fungi
In vitro study
medicine.disease
biology.organism_classification
Bacterial DNA
Maternal deprivation
Nonhuman
Drug efficacy
Viscera
030104 developmental biology
Fungal DNA
chemistry
Growth inhibition
Dysbiosis
Rat
Antibacterial activity
Colorectal disease
Controlled study
Internal transcribed spacer 1
Mycobiome
Zdroj: Neurogastroenterology and Motility, 6, 30
Neurogastroenterology and motility, 30(6). Wiley-Blackwell
ISSN: 1350-1925
Popis: Background: Irritable bowel syndrome (IBS) is a common gastrointestinal disorder associated with altered gastrointestinal microflora and increased nociception to colonic distension. This visceral hypersensitivity can be reversed in our rat maternal separation model by fungicides. Menthacarin® is a proprietary combination of essential oils from Mentha x piperita L. and Carum carvi. Because these oils exhibit antifungal and antibacterial properties, we investigated whether Menthacarin® can reverse existing visceral hypersensitivity in maternally separated rats. Methods: In non-handled and maternally separated rats, we used the visceromotor responses to colorectal distension as measure for visceral sensitivity. We evaluated this response before and 24 hours after water-avoidance stress and after 7 days treatment with Menthacarin® or control. The pre- and post-treatment mycobiome and microbiome were characterized by sequencing of fungal internal transcribed spacer 1 (ITS-1) and bacterial 16s rDNA regions. In vitro antifungal and antimicrobial properties of Menthacarin® were studied with radial diffusion assay. Key Results: Menthacarin® inhibited in vitro growth of yeast and bacteria. Water-avoidance caused visceral hypersensitivity in maternally separated rats, and this was reversed by treatment. Multivariate analyses of ITS-1 and 16S high throughput data showed that maternal separation, induced changes in the myco- and microbiome. Menthacarin® treatment of non-handled and maternally separated rats shifted the mycobiomes to more similar compositions. Conclusions & Inferences: The development of visceral hypersensitivity in maternally separated rats and the Menthacarin®-mediated reversal of hypersensitivity is associated with changes in the mycobiome. Therefore, Menthacarin® may be a safe and effective treatment option that should be tested for IBS. © 2018 The Authors. Neurogastroenterology & Motility Published by John Wiley & Sons Ltd. Chemicals / CAS: decanoic acid, 334-48-5, 3398-75-2; fluconazole, 86386-73-4; octanoic acid, 124-07-2, 1984-06-1, 74-81-7; peppermint oil, 8006-90-4; streptomycin, 57-92-1
Databáze: OpenAIRE
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