Generation of Embryonic Stem Cell Lines from Immature Rabbit Ovarian Follicles
| Autor: | Yuta Kida, Yuta Onodera, John P. Frampton, Yoshihiko Hosoi, Takeshi Teramura, Naoto Fukunaga, Hiroki Izumi, Hironobu Sugimoto, Kanji Fukuda, Makiko Kawakami, Toshiyuki Takehara, Miho Nakano |
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| Rok vydání: | 2013 |
| Předmět: |
Homeobox protein NANOG
Cellular differentiation Cell Culture Techniques Mice SCID Embryoid body Biology Cell Line Embryo Culture Techniques Mice Ovarian Follicle Original Research Reports SOX2 medicine Animals Ovarian follicle Induced pluripotent stem cell Embryoid Bodies reproductive and urinary physiology urogenital system Cell Differentiation Cell Biology Hematology Antigens Differentiation Embryonic stem cell Cell biology In vitro maturation Blastocyst medicine.anatomical_structure embryonic structures Immunology Oocytes Female Rabbits biological phenomena cell phenomena and immunity Transcriptome Developmental Biology |
| Zdroj: | Stem Cells and Development. 22:928-938 |
| ISSN: | 1557-8534 1547-3287 |
| Popis: | In mammalian ovaries, many immature follicles remain after the dominant follicles undergo ovulation. Here we report the successful production of rabbit embryonic stem cells (ESCs) from oocytes produced by in vitro culture of immature follicles and subsequent in vitro maturation treatment. In total, we obtained 53 blastocysts from oocytes that received intracytoplasmic sperm injection followed by in vitro culture. Although only weak expression of POU5f1 was observed in the inner cell masses of in-vitro-cultured follicle-derived embryos, repeated careful cloning enabled establishment of 3 stable ESC lines. These ESC lines displayed the morphological characteristics of primed pluripotent stem cells. The ESC lines also expressed the pluripotent markers Nanog, POU5f1, and Sox2. Further, these ESCs could be differentiated into each of the 3 different germ layers both in vitro and in vivo. These results demonstrate that immature follicles from rabbits can be used to generate ESCs. Moreover, the use of rabbit oocytes as a cell source provides an experimental system that closely matches human reproductive and stem cell physiology. |
| Databáze: | OpenAIRE |
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