Evidence that human blastomere cleavage is under unique cell cycle control
| Autor: | Ann A. Kiessling, Dimitris Loutradis, R. Bletsa, Christina Mara, K. Kallianidis, B. Desmarais |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
DNA Replication
Male Pluripotent Stem Cells Blastomeres Cell cycle checkpoint Cell division Microarray CLOCK Cell Cycle Proteins Myc Proto-Oncogene Mas Obstetrics and Gynaecology Genetics Human embryo Humans Genetics(clinical) RNA Messenger Cell Cycle Protein Induced pluripotent stem cell Retinoblastoma gene Human blastomere Genetics (clinical) UHRF2 Oligonucleotide Array Sequence Analysis biology Cell Cycle Totipotent Obstetrics and Gynecology General Medicine Blastomere Circadian oscillators Fibroblasts Cell cycle Circadian Rhythm Cell biology Wee1 Reproductive Medicine biology.protein Female Growth factor independent Cell Division Developmental Biology |
| Zdroj: | Journal of Assisted Reproduction and Genetics |
| ISSN: | 1573-7330 1058-0468 |
| Popis: | Purpose To understand the molecular pathways that control early human embryo development. Methods Improved methods of linear amplification of mRNAs and whole human genome microarray analyses were utilized to characterize gene expression in normal appearing 8-Cell human embryos, in comparison with published microarrays of human fibroblasts and pluripotent stem cells. Results Many genes involved in circadian rhythm and cell division were over-expressed in the 8-Cells. The cell cycle checkpoints, RB and WEE1, were silent on the 8-Cell arrays, whereas the recently described tumor suppressor, UHRF2, was up-regulated >10-fold, and the proto-oncogene, MYC, and the core element of circadian rhythm, CLOCK, were elevated up to >50-fold on the 8-Cell arrays. Conclusions The canonical G1 and G2 cell cycle checkpoints are not active in totipotent human blastomeres, perhaps replaced by UHRF2, MYC, and intracellular circadian pathways, which may play important roles in early human development. Electronic supplementary material The online version of this article (doi:10.1007/s10815-009-9306-x) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink