A simple method to eliminate mycoplasma from cell cultures
Autor: | Diana Lia Vullo, C. E. Coto, Silvia Coronato |
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Rok vydání: | 1994 |
Předmět: |
medicine.drug_class
Antibiotics Minocycline Mycoplasmataceae macromolecular substances medicine.disease_cause Microbiology Mycoplasma Kanamycin Cricetinae Culture Techniques Virology Chlorocebus aethiops Tumor Cells Cultured medicine Animals Humans Incubation Cells Cultured Roxithromycin biology Drug Resistance Microbial biology.organism_classification Eukaryotic Cells Vero cell Drug Therapy Combination Tylosin Mycoplasma orale medicine.drug |
Zdroj: | Journal of Virological Methods. 46:85-94 |
ISSN: | 0166-0934 |
DOI: | 10.1016/0166-0934(94)90018-3 |
Popis: | The efficacy of several antibiotic treatments to eliminate mycoplasma from Vero cells contaminated chronically with Mycoplasma orale II were tested. Minocyclin, Kanamycin, Tylosine and Roxitromycin, at non cytotoxic concentrations, were assayed alone or in different combinations. Mycoplasma contamination was effectively eradicated without recurrence once the following regimen was applied: Incubation of contaminated cells with Tylosine (250 micrograms/ml) for 12 days followed by incubation with Minocycline (5 micrograms/ml) for 10 days. This treatment was not deleterious for cell growth, it was effective after only one application and it was successful to eradicate mycoplasma from other contaminated eukaryotic continuous cell lines. |
Databáze: | OpenAIRE |
Externí odkaz: |
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