A Protodermal miR394 Signal Defines a Region of Stem Cell Competence in the Arabidopsis Shoot Meristem
| Autor: | Matthew R. Tucker, Marja C.P. Timmermans, Anna L. Holt, Thomas Laux, Ignacio Rubio-Somoza, Elise J. Tucker, Steffen Knauer, Marie Javelle, Melanie Pisch, Annika Hinze |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Pluripotent Stem Cells
Plant stem cell Cellular differentiation Meristem Arabidopsis Apical cell General Biochemistry Genetics and Molecular Biology Gene Expression Regulation Plant Induced pluripotent stem cell Molecular Biology Genetics Homeodomain Proteins biology Arabidopsis Proteins F-Box Proteins fungi food and beverages Cell Differentiation Cell Biology biology.organism_classification Cell biology ABC model of flower development MicroRNAs Stem cell Signal Transduction Developmental Biology |
| Zdroj: | Developmental Cell. 24(2):125-132 |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2012.12.009 |
| Popis: | A long-standing question in plants and animals is how spatial patterns are maintained within stem cell niches despite ongoing cell divisions. Here we address how, during shoot meristem formation in Arabidopsis thaliana, the three apical cell layers acquire stem cell identity. Using a sensitized mutant screen, we identified miR394 as a mobile signal produced by the surface cell layer (the protoderm) that confers stem cell competence to the distal meristem by repressing the F box protein LEAF CURLING RESPONSIVENESS. This repression is required to potentiate signaling from underneath the stem cells by the transcription factor WUSCHEL, maintaining stem cell pluripotency. The interaction of two opposing signaling centers provides a mechanistic framework of how stem cells are localized at the tip of the meristem. Although the constituent cells change, the surface layer provides a stable point of reference in the self-organizing meristem. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|