Effects of temperature and water activity on FUM2 and FUM21 gene expression and fumonisin B production in Fusarium verticillioides
| Autor: | Irene Lazzaro, Adriano Marocco, Rosalia Ferracane, Paola Battilani, Alberto Ritieni, Antonia Susca, Giuseppina Mulè |
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| Rok vydání: | 2012 |
| Předmět: |
Fusarium
biology Water activity food and beverages Plant Science Horticulture biosynthetic pathway biology.organism_classification mycotoxin Real-time RT-PCR Incubation period Microbiology chemistry.chemical_compound chemistry Gene expression Fumonisin FUM cluster Biosynthetic pathway . Ecology . FUM cluster . Mycotoxin . Real Time RT-PCR ecology Settore AGR/12 - PATOLOGIA VEGETALE Mycotoxin Agronomy and Crop Science Gene Incubation |
| Zdroj: | European journal of plant pathology 134 (2012): 685–695. doi:10.1007/s10658-012-0045-y info:cnr-pdr/source/autori:Lazzaro I., Susca A., Mulè G., Ritieni A., Ferracane R., Marocco A., Battilani P/titolo:Effects of temperature and water activity on FUM2 and FUM21 gene expression and fumonisin B production in Fusarium verticillioides/doi:10.1007%2Fs10658-012-0045-y/rivista:European journal of plant pathology/anno:2012/pagina_da:685/pagina_a:695/intervallo_pagine:685–695/volume:134 |
| ISSN: | 1573-8469 0929-1873 |
| DOI: | 10.1007/s10658-012-0045-y |
| Popis: | The fungus Fusarium verticillioides is a pathogen of maize and can produce the mycotoxins fumonisins. The present work examined the effect of abiotic factors on the expression of two fumonisin biosynthetic genes (FUM2 and FUM21) and fumonisin production in the fungus. Specifically, the effects of water activity (0.901-0.990), temperature (20-30°C) and incubation time (7-21 days) were analysed in cultures of F. verticillioides strains ITEM 10027 and ITEM 1744. Fumonisin B were measured through liquid chromatography tandem mass spectrometry and gene expression was quantified with relative real-time PCR, using the Livak method. Fumonisin production increased with incubation time up to 21 days and the transcription of both genes was highest at 14 days; however intraspecific variability was observed. FUM2 and FUM21 expression was positively correlated to fumonisin synthesis (p |
| Databáze: | OpenAIRE |
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