Mass Spectrometry-Based Metabolomics to Investigate the Effect of Mechanical Shaking on Sauvignon Blanc Berry Metabolism
| Autor: | Brigitte Heike Schwendel, Farhana R. Pinu, Erica Zarate, Kyung Whan Bang, Angus C. Grey, George Guo, Praju Vikas Anekal |
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| Rok vydání: | 2021 |
| Předmět: |
biology
Chemistry food and beverages Primary metabolite Lipid metabolism General Chemistry Berry biology.organism_classification Gas Chromatography-Mass Spectrometry Mass Spectrometry Veraison Metabolomics Fruit Vitis Botrytis Food science Gas chromatography General Agricultural and Biological Sciences Secondary metabolism New Zealand Botrytis cinerea |
| Zdroj: | Journal of Agricultural and Food Chemistry. 69:4918-4933 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | Previous commercial studies carried out in New Zealand showed that mechanical shaking significantly reduced the incidence of Botrytis cinerea infection in wine grapes. However, the reasons behind this reduction are not well understood. Here, we employed a metabolomics approach to gain insights into the biochemical changes that occur in grape berries due to mechanical shaking. Berry samples were analyzed using three different analytical approaches including gas chromatography and mass spectrometry (MS), liquid chromatography and MS, and imaging mass spectrometry (IMS). Combined data provided a comprehensive overview of metabolic changes in grape berry, indicating the initiation of different stress mitigation strategies to overcome the effect of mechanical shaking. Berry primary metabolism was distinctly altered in the green berries in response to mechanical shaking, while secondary metabolism significantly changed in berries collected after veraison. Pathway analysis showed upregulation of metabolites related to nitrogen and lipid metabolism in the berries from shaken vines when compared with controls. From IMS data, we observed an accumulation of different groups of metabolites including phenolic compounds and amino and fatty acids in the areas near to the skin of berries from shaken vines. This observation suggests that mechanical shaking caused an accumulation of these metabolites, which may be associated with the formation of a protective barrier, leading to the reduction in B. cinerea infection in berries from mechanically shaken vines. |
| Databáze: | OpenAIRE |
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