Formulation, Characterisation and Evaluation of the Antihypertensive Peptides, Isoleucine-Proline-Proline and Leucine-Lysine-Proline in Chitosan Nanoparticles Coated with Zein for Oral Drug Delivery
| Autor: | Minna Khalid Danish, John P. Gleeson, David J. Brayden, Hugh J. Byrne, Jesus M. Frías, Sinéad M. Ryan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Proline
Zein Administration Oral Isoleucine-Proline-Proline (IPP) Leucine-Lysine-Proline (LKP) oral peptide delivery zein antihypertensive peptides Catalysis Inorganic Chemistry Leucine Rats Inbred SHR Medicine and Health Sciences Animals Humans Physical and Theoretical Chemistry Particle Size Molecular Biology Spectroscopy Antihypertensive Agents Medicinal and Pharmaceutical Chemistry Chitosan Drug Carriers Lysine Organic Chemistry General Medicine Computer Science Applications Rats Nanoparticles Caco-2 Cells Peptides Oligopeptides |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 19; Pages: 11160 Articles |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms231911160 |
| Popis: | Isoleucine-Proline-Proline (IPP) and Leucine-Lysine-Proline (LKP) are food-derived tripeptides whose antihypertensive functions have been demonstrated in hypertensive rat models. However, peptides display low oral bioavailability due to poor intestinal epithelial permeability and instability. IPP and LKP were formulated into nanoparticles (NP) using chitosan (CL113) via ionotropic gelation and then coated with zein. Following addition of zein, a high encapsulation efficiency (EE) (>80%) was obtained for the NP. In simulated gastric fluid (SGF), 20% cumulative release of the peptides was achieved after 2 h, whereas in simulated intestinal fluid (SIF), ~90% cumulative release was observed after 6 h. Higher colloidal stability (39–41 mV) was observed for the coated NP compared to uncoated ones (30–35 mV). In vitro cytotoxicity studies showed no reduction in cellular viability of human intestinal epithelial Caco-2 and HepG2 liver cells upon exposure to NP and NP components. Administration of NP encapsulating IPP and LKP by oral gavage to spontaneously hypertensive rats (SHR) attenuated systolic blood pressure (SBP) for 8 h. This suggests that the NP provide appropriate release to achieve prolonged hypotensive effects in vivo. In conclusion, chitosan-zein nanoparticles (CZ NP) have potential as oral delivery system for the encapsulation of IPP and LKP. |
| Databáze: | OpenAIRE |
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