Rapid detection of human blood in triatomines (kissing bugs) utilizing a lateral flow immunochromatographic assay - A pilot study
| Autor: | Nicole Behrens-Bradley, Nafees Ahmad, Shannon Smith, Patricia L. Dorn, Finn McCants, Stephen A. Klotz, Sarah A. Hamer, Norman Beatty, Justin O. Schmidt, Maria Love |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
Chagas disease Veterinary medicine Time Factors lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 030231 tropical medicine lcsh:QR1-502 Positive control Negative control Pilot Projects Biology Triatoma rubida Rapid detection lcsh:Microbiology Feces Mice 03 medical and health sciences 0302 clinical medicine Reference Values medicine Animals Humans Triatominae human blood detection blood sources Immunoassay Human blood digestive oral and skin physiology Reproducibility of Results Original Articles Reference Standards medicine.disease biology.organism_classification Blood triatomine kissing bug |
| Zdroj: | Memórias do Instituto Oswaldo Cruz, Volume: 114, Article number: e190047, Published: 30 MAY 2019 Memórias do Instituto Oswaldo Cruz., Vol 114, Iss 0 Memórias do Instituto Oswaldo Cruz |
| Popis: | BACKGROUND DNA- and proteomics-based techniques are currently used to identify a triatomine human blood meal. These methods are time consuming, require access to laboratories with sophisticated equipment, and trained personnel. OBJECTIVES We tested a rapid and specific immunochromatographic assay (that detects human blood in forensic samples) to determine if human blood was present in triatomines and their fecal excreta. METHODS We fed Triatoma rubida human blood (positive control) or mouse blood (negative control) and performed the assay on the abdominal contents and fecal excreta. Triatomine field specimens collected in and around human habitations and excreta were also tested. FINDINGS The assay was positive in triatomines fed human blood (N = 5/5) and fecal excreta from bugs known to have ingested human blood (N = 5/5). Bugs feeding on mice (N = 15/15) and their fecal excreta (N = 8/8) were negative for human blood. Human blood was detected in 47% (N = 23/49) triatomines, representing six different species, collected in the field. MAIN CONCLUSIONS The pilot study shows that this rapid and specific test may have applications in triatomine research. Further study is needed to determine the sensitivity of this assay compared to other well-established techniques, such as DNA- and proteomics-based methodologies and the assay’s application in the field. |
| Databáze: | OpenAIRE |
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