Elemental content in the nucleus and in yolk and yolk-free cytoplasm of developingRana pipiens oocytes: An X-ray microanalysis study
| Autor: | Thomas B. Pool, Nancy K. R. Smith, D. R. L. Labadie, Ivan L. Cameron |
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| Rok vydání: | 1981 |
| Předmět: |
Cytoplasm
food.ingredient Physiology Clinical Biochemistry Ovary Biology Microanalysis Rana food Yolk medicine Animals Platelet skin and connective tissue diseases Ovum Cell Nucleus Rana pipiens Sodium Cell Biology Oocyte medicine.anatomical_structure Biochemistry Oocytes Potassium Biophysics Female sense organs Nucleus Electron Probe Microanalysis |
| Zdroj: | Journal of Cellular Physiology. 109:91-97 |
| ISSN: | 1097-4652 0021-9541 |
| DOI: | 10.1002/jcp.1041090111 |
| Popis: | Energy dispersive x-ray microanalysis measured the Na, K, Cl, P, Mg, S, and Ca contents (mM/kg dry weight) in the nucleus, yolk-free cytoplasm, and yolk platelets of Rana pipiens oocytes quick frozen in the ovary. The data revealed that significant content changes occur in frog oocytes during intraovarian growth. All elements but Ca changed in content in the nucleus and cytoplasm, while in the yolk platelet only Na content did not change. A nucleus to cytoplasm K gradient develops and increases in magnitude during oocyte growth. The data from this and previous reports lead to the hypothesis that intra-oocytic water and elements undergo changes in state during oocyte growth and that three subcellular Na compartments exist. |
| Databáze: | OpenAIRE |
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