trans Activation of Gene Expression by v-myb
| Autor: | Ibanez, C E, Lipsick, J S |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
animal structures
Transcription Genetic Retroviridae Proteins Oncogenic fungi DNA Recombinant Coturnix Oncogene Proteins Viral Oncogenes Cell Biology In Vitro Techniques Regulatory Sequences Nucleic Acid Oncogene Proteins v-myb Cell Line DNA-Binding Proteins Gene Expression Regulation Animals Molecular Biology Cell Division Research Article Transcription Factors |
| Zdroj: | Molecular and Cellular Biology. 10:2285-2293 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.10.5.2285-2293.1990 |
| Popis: | The v-myb oncogene causes acute myelomonocytic leukemia in chickens and transforms avian myeloid cells in vitro. Its product, p48v-myb, is a short-lived nuclear protein which binds DNA. We demonstrate that p48v-myb can function as a trans activator of gene expression in transient DNA transfection assays. trans activation requires the highly conserved amino-terminal DNA-binding domain and the less highly conserved carboxyl-terminal domain of p48v-myb, both of which are required for transformation. Multiple copies of a consensus sequence for DNA binding by p48v-myb inserted upstream of a herpes simplex virus thymidine kinase promoter are strongly stimulatory for transcriptional activation by a v-myb-VP16 fusion protein but not by p48v-myb itself, suggesting that the binding of p48v-myb to DNA may not be sufficient for trans activation. |
| Databáze: | OpenAIRE |
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