Black bean extract ameliorates liver fibrosis in rats with CCl4-induced injury
| Autor: | Vicente Morales-Oyarvide, Gustavo E. Guajardo-Salinas, Netzahualcóyotl Arroyo-Currás, Víctor Javier Lara-Díaz, Beiman G Cano, Jorge E. Moreno-Cuevas, Francisco Javier Gálvez-Gastélum, Alberto López-Reyes, Luis Alonso Morales-Garza, Jose Francisco Islas, Guillermo Grijalva |
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| Rok vydání: | 2008 |
| Předmět: |
Collagen Type IV
Liver Cirrhosis Male Pathology medicine.medical_specialty Cirrhosis Specialties of internal medicine CCL4 Pharmacology medicine.disease_cause Collagen Type I Antioxidants chronic diseases chemistry.chemical_compound Fibrosis medicine Animals oxidative stress Rats Wistar black bean extract Carbon Tetrachloride liver fibrosis Flavonoids Liver injury Hepatology Plant Extracts business.industry cirrhosis Fabaceae General Medicine medicine.disease Rats Disease Models Animal Liver RC581-951 chemistry Carbon tetrachloride Quercetin business Hepatic fibrosis Oxidative stress Phytotherapy |
| Zdroj: | Annals of Hepatology, Vol 7, Iss 2, Pp 130-135 (2008) |
| ISSN: | 1665-2681 |
| DOI: | 10.1016/s1665-2681(19)31868-x |
| Popis: | We assessed the anti-fibrotic effects of methanolic black bean extract antioxidants in a carbon tetrachlo- ride (CCl 4 ) liver injury model in rats. Experimentally intoxicated animals received CCl 4 for eight weeks, the reference and test groups received daily intragastric quercetin or daily intragastric black bean extract. Liv- er fibrosis was assessed and quantified using morpho- metric analysis. Expression of fibrosis related genes was measured by real time RT-PCR. Qualitative and quantitative histological analysis showed that adminis- tration of 70 mg/kg b.w. of black bean extract reduced hepatic fibrosis index by 18% compared to positive controls (P 0.006), as a result of a decrease in type I (44.3% less, P 0.03) and type IV (68.9% less, P 0.049) collagen gene expression compared to CCl 4 -injured and Quercetin treated rats. In conclusion, we provide evidence that this methanol black bean extract amelio- rates liver fibrosis and types I and IV collagen gene ex- pression, in the animal model used. Practical applications: The compounds contained in this black bean extract exhibited strong antifibrotic ef- fects in the CCl 4 chronic liver injury model used; con- sidering that this compounds are contained in a legu- minous that has been used in human diet for a long time, their toxic potential should be very low, and this characteristic should favor their potential use in some other chronic or degenerative states that include an in- crease in inflammation and oxidative burst in their pathogenesis. Another possible application of this kind of extract could be its use as an antimicrobial or even antiparasitic therapeutic agent, although it is purely speculative. |
| Databáze: | OpenAIRE |
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