Metallo-β-lactamase-producing Clinical Isolates from Patients of a Tertiary Care Hospital
| Autor: | AS Damle, Durgesh G. Deshmukh, JK Bajaj, NS Patwardhan, Jayshree B. Bhakre |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Bacilli
Imipenem lcsh:Medicine chemical and pharmacologic phenomena Biology Metallo β lactamase Microbiology law.invention Antibiotic resistance law medicine polycyclic compounds non-fermenting bacilli lcsh:R biochemical phenomena metabolism and nutrition biology.organism_classification bacterial infections and mycoses Enterobacteriaceae Intensive care unit Multiple drug resistance metallo-β-lactamases Original Article Analytical profile index medicine.drug enterobacteriaceae imipenem |
| Zdroj: | Journal of Laboratory Physicians, Vol 3, Iss 02, Pp 093-097 (2011) Journal of Laboratory Physicians |
| ISSN: | 0974-7826 0974-2727 |
| Popis: | Background: The growing increase in the rates of antibiotic resistance is a major cause for concern in both non-fermenting bacilli and isolates of the Enterobacteriaceae family. β-lactams have been the mainstay of treatment for serious infections, and the most active of these are the carbapenems. Acquired metallo-β-lactamases (MBL) have recently emerged as one of the most worrisome resistance mechanisms owing to their capacity to hydrolyze all β-lactams, including carbapenems. We have undertaken this investigation to ascertain the prevalence of MBL-producing non-fermenting bacilli and Enterobacteriaceae. Materials and Methods: The study was conducted over a period of 4 months in a 1200-bedded teaching hospital. Isolates included in the study were screened for imipenem resistance both by conventional methods and mini analytical profile index (miniAPI). The isolates that showed imipenem resistance were tested for MBL production by imipenem (IMP)-ethylenediaminetetraacetic acid combined disc test. Imipenem-resistant non-MBL isolates also tested for Modified Hodge test and AmpC β-lactamases production to detect other mechanisms of carbapenem resistance. Results: Of 638 gram negative bacilli isolates and 3.39% showed imipenem resistance, 2.9% showed MBL production, of which 1.7% were non-fermenters and 1.25% were Enterobacteriaceae, 0.3% showing non-MBL KPC carbapenemas. Most isolates were from the intensive care unit and from post-operative patients. Our findings show that there are significant numbers of isolates having MBL production along with multidrug resistance. There is a need for active surveillance to detect MBL producers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|