Nutrigenomics in honey bees: digital gene expression analysis of pollen's nutritive effects on healthy and varroa-parasitized bees

Autor: Christelle Dantec, Yves Le Conte, Hughes Parrinello, Cédric Alaux
Přispěvatelé: Abeilles et Environnement (AE), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), This work was funded by a BEEDOC grant (FP7, RTD REG/E.4 (2009)D/561221) and C. Alaux was supported by an INRA young researcher position (INRA SPE department)., Alaux, Cédric, BMC, Ed., Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Avignon Université (AU)-Institut National de la Recherche Agronomique (INRA)
Rok vydání: 2011
Předmět:
0106 biological sciences
HOMEOSTASIS
HYMENOPTERA
NUTRIGENOMIQUE
SUSCEPTIBILITY
medicine.disease_cause
ACARI
01 natural sciences
Transcriptome
HONEYBEE
Nutrigenomics
SANTE
Abdomen
2. Zero hunger
0303 health sciences
biology
TOR Serine-Threonine Kinases
food and beverages
VARROA DESTRUCTOR
Bees
GENOMICS TOOL
Worker bee
protéine
behavior and behavior mechanisms
[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
Pollen
Varroa
NUTRITION
HEALTH
APIDAE
Biotechnology
Signal Transduction
Research Article
lcsh:QH426-470
lcsh:Biotechnology
Varroidae
Longevity
Carbohydrates
malnutrition
TRANSCRIPTOME VARIATION
complex mixtures
NUTRIGENOMICS
APIS MELLIFERA
ABEILLE DOMESTIQUE
TOLERANCE
03 medical and health sciences
protéine végétale
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
lcsh:TP248.13-248.65
Botany
medicine
Genetics
Animals
030304 developmental biology
homéostasie
fungi
Honey bee
biology.organism_classification
010602 entomology
lcsh:Genetics
Varroa destructor
Bee pollen
Antimicrobial Cationic Peptides
Zdroj: BMC Genomics
BMC Genomics, Vol 12, Iss 1, p 496 (2011)
BMC Genomics, BioMed Central, 2011, 12 (1), pp.496. ⟨10.1186/1471-2164-12-496⟩
BMC Genomics (12), 14 p. (12:496). (2011)
BMC Genomics, 2011, 12 (1), pp.496. ⟨10.1186/1471-2164-12-496⟩
ISSN: 1471-2164
DOI: 10.1186/1471-2164-12-496⟩
Popis: Background Malnutrition is a major factor affecting animal health, resistance to disease and survival. In honey bees (Apis mellifera), pollen, which is the main dietary source of proteins, amino acids and lipids, is essential to adult bee physiological development while reducing their susceptibility to parasites and pathogens. However, the molecular mechanisms underlying pollen's nutritive impact on honey bee health remained to be determined. For that purpose, we investigated the influence of pollen nutrients on the transcriptome of worker bees parasitized by the mite Varroa destructor, known for suppressing immunity and decreasing lifespan. The 4 experimental groups (control bees without a pollen diet, control bees fed with pollen, varroa-parasitized bees without a pollen diet and varroa-parasitized bees fed with pollen) were analyzed by performing a digital gene expression (DGE) analysis on bee abdomens. Results Around 36, 000 unique tags were generated per DGE-tag library, which matched about 8, 000 genes (60% of the genes in the honey bee genome). Comparing the transcriptome of bees fed with pollen and sugar and bees restricted to a sugar diet, we found that pollen activates nutrient-sensing and metabolic pathways. In addition, those nutrients had a positive influence on genes affecting longevity and the production of some antimicrobial peptides. However, varroa parasitism caused the development of viral populations and a decrease in metabolism, specifically by inhibiting protein metabolism essential to bee health. This harmful effect was not reversed by pollen intake. Conclusions The DGE-tag profiling methods used in this study proved to be a powerful means for analyzing transcriptome variation related to nutrient intake in honey bees. Ultimately, with such an approach, applying genomics tools to nutrition research, nutrigenomics promises to offer a better understanding of how nutrition influences body homeostasis and may help reduce the susceptibility of bees to (less virulent) pathogens.
Databáze: OpenAIRE
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