Genome-wide mRNA and miRNA analysis of peripheral blood mononuclear cells (PBMC) reveals different miRNAs regulating HIV/HCV co-infection
| Autor: | Priyanka Gupta, Eugenia Vispo, Murray J. Cairns, Jing Qin Wu, Bing Liu, Adam P. Carroll, Belinda J. Goldie, Nitin K. Saksena, Vicente Soriano |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Microarray Hepacivirus Hepatitis C virus HIV Infections medicine.disease_cause Peripheral blood mononuclear cell Virology microRNA medicine Humans Aged Genome biology Coinfection Gene Expression Profiling virus diseases Hepatitis C Middle Aged biology.organism_classification medicine.disease Gene expression profiling MicroRNAs Case-Control Studies Immunology HIV-1 Leukocytes Mononuclear Female |
| Zdroj: | Virology. :336-349 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2013.12.026 |
| Popis: | Co-infection with human immunodeficiency virus (HIV) and hepatitis C virus (HCV) is common due to shared transmission routes. The genomic basis of HIV/HCV co-infection and its regulation by microRNA (miRNA) is unknown. Therefore, our objective was to investigate genome-wide mRNA expression and its regulation by miRNA in primary PBMCs derived from 27 patients (5 HCV - mono-infected, 5 HIV-mono-infected, 12 HCV/HIV co-infected, and 5 healthy controls). This revealed 27 miRNAs and 476 mRNAs as differentially expressed (DE) in HCV/HIV co-infection when compared to controls (adj p0.05). Our study shows the first evidence of miRNAs specific for co-infection, several of which are correlated with key gene targets demonstrating functional relationships to pathways in cancer, immune-function, and metabolism. Notable was the up regulation of HCV-specific miR-122 in co-infection (FC50, p=4.02E-06), which may have clinical/biological implications. |
| Databáze: | OpenAIRE |
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