Multimodal Probes: Superresolution and Transmission Electron Microscopy Imaging of Mitochondria, and Oxygen Mapping of Cells, Using Small-Molecule Ir(III) Luminescent Complexes
| Autor: | Luke K. McKenzie, Stanley W. Botchway, Michael D. Ward, Ahmet J. Cankut, Julia A. Weinstein, Jonathan R. Shewring, Elizabeth Edwards, Bethany J. Crowston |
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| Rok vydání: | 2017 |
| Předmět: |
Confocal
Iridium 010402 general chemistry 01 natural sciences law.invention Inorganic Chemistry Microscopy Electron Transmission Coordination Complexes law Microscopy Humans Physical and Theoretical Chemistry Luminescent Agents Microscopy Confocal 010405 organic chemistry Chemistry QH Optical Imaging Small molecule Mitochondria 0104 chemical sciences Oxygen Transmission electron microscopy Biophysics Ultrastructure Electron microscope Luminescence Phosphorescence HeLa Cells |
| Zdroj: | Inorganic Chemistry. 56:15259-15270 |
| ISSN: | 1520-510X 0020-1669 |
| DOI: | 10.1021/acs.inorgchem.7b02633 |
| Popis: | We describe an Ir(III)-based small-molecule, multimodal probe for use in both light and electron microscopy. The direct correlation of data between light- and electron-microscopy-based imaging to investigate cellular processes at the ultrastructure level is a current challenge, requiring both dyes that must be brightly emissive for luminescence imaging and scatter electrons to give contrast for electron microscopy, at a single working concentration suitable for both methods. Here we describe the use of Ir(III) complexes as probes that provide excellent image contrast and quality for both luminescence and electron microscopy imaging, at the same working concentration. Significant contrast enhancement of cellular mitochondria was observed in transmission electron microscopy imaging, with and without the use of typical contrast agents. The specificity for cellular mitochondria was also confirmed with MitoTracker using confocal and 3D-structured illumination microscopy. These phosphorescent dyes are part of a very exclusive group of transition-metal complexes that enable imaging beyond the diffraction limit. Triplet excited-state phosphorescence was also utilized to probe the O2 concentration at the mitochondria in vitro, using lifetime mapping techniques. |
| Databáze: | OpenAIRE |
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