Protein Bead Array for the Detection of HIV-1 Antibodies from Fresh Plasma and Dried-Blood-Spot Specimens
| Autor: | Alexandre Martel, Paul Sandstrom, John E. Kim, Alice Sherring, Sylvie Faucher, Michèle Bergeron, Francis Mandy, Laurie Malloch, T. Ding |
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| Rok vydání: | 2004 |
| Předmět: |
Clinical Biochemistry
Protein Array Analysis Human immunodeficiency virus (HIV) HIV Antibodies Bead medicine.disease_cause Immunoenzyme Techniques Plasma Suspension array technology medicine Humans Blood Specimen Collection Chromatography biology medicine.diagnostic_test Chemistry Biochemistry (medical) AIDS Serodiagnosis Virology Dried blood spot visual_art Immunoassay HIV-1 biology.protein visual_art.visual_art_medium Antibody Bead array Blood sampling |
| Zdroj: | Clinical Chemistry. 50:1250-1253 |
| ISSN: | 1530-8561 0009-9147 |
| Popis: | Simultaneous multianalyte immunoassays offer advantages, including reduction of technical operations, execution time, and overall costs. The suspension array technology (SAT), which uses microfluorospheres with flow cytometry, is a multianalyte immunoassay that requires small specimen volumes, such as those obtained by less-invasive blood sampling approaches such as heel or finger sticks, as well as those obtained in pediatric specimens. The dried-blood-spot (DBS) technology has become an important screening tool for clinical and epidemiologic testing (1)(2)(3). It is particularly convenient in rural, resource-limited settings where trained personnel and adequate facilities for blood collection, processing, transport, and storage may not be available. This approach, however, provides limited specimen volume (∼5–6 μL of serum/6-mm punch) (2). Fortunately, SAT is well suited to support such a format (4)(5). The range of applications for SAT includes antibody, oligonucleotide, peptide, and protein bead arrays (PBAs) (4)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17). PBAs have been used for the simultaneous detection of serum antibodies to infectious agents for systemic candidiasis (18), herpes viruses (19), measles, mumps (20), and HIV (21)(22). The successful detection of HIV-1 serum antibodies by a four-protein bead array was first reported by Scillian et al. (21), who used beads of various sizes and proteins noncovalently coupled to beads. Reagents are now available that permit covalent coupling of proteins to beads and simultaneous analysis of up to 100 color-coded bead sets in a high-throughput, 96-well plate format. In addition, a new generation of flow cytometers compatible with SAT has recently been introduced, e.g., the Luminex-100 System (Luminex Corp.) and the FACSArray (BD Biosciences). We have developed and evaluated a HIV-1 PBA for the rapid detection of antibodies to … |
| Databáze: | OpenAIRE |
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