Multichannel Microchip Electrospray Mass Spectrometry

Autor:	Nicol E. McGruer, Paul M. Zavracky, František Foret, Barry L. Karger, Yuriy M. Dunayevskiy, Qifeng Xue
Rok vydání:	1997
Předmět:	Detection limit Electrospray Chromatography Chemistry Capillary action Electrospray ionization Analytical chemistry Proteins Mass spectrometry Isotropic etching Mass Spectrometry Analytical Chemistry Volumetric flow rate Microcomputers Miniaturization Peptides
Zdroj:	Analytical Chemistry. 69:426-430
ISSN:	1520-6882 0003-2700
DOI:	10.1021/ac9607119
Popis:	Microfabricated multiple-channel glass chips were successfully interfaced to an electrospray ionization mass spectrometer (ESI-MS). The microchip device was fabricated by standard photolithographic, wet chemical etching, and thermal bonding procedures. A high voltage was applied individually from each buffer reservoir for spraying sample sequentially from each channel. With the sampling orifice of the MS grounded, it was found that a liquid flow of 100-200 nL/min was necessary to maintain a stable electrospray. The detection limit of the microchip MS experiment for myoglobin was found to be lower than 6 x 10(-8) M. Samples in 75% methanol were successfully analyzed with good sensitivity, as were aqueous samples. The parallel mutliple-channel microchip system allowed ESI-MS analysis of different samples of standard peptides and proteins in one chip.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::876c36d3ce39c46e1b0b06b38b91e7ea https://doi.org/10.1021/ac9607119 Zobrazit plný text záznamu