Proteomic Analysis of Differential Protein Expression of Achilles Tendon in a Rabbit Model by Two-Dimensional Polyacrylamide Gel Electrophoresis at 21 Days Postoperation
| Autor: | Nuerai Shawutali, Bin Tang, Ainuer Jialili, Jingping Bai, Kayrat Aldyarhan, Darebai Redati, Gulnur Sabirhazi, Jiasharete Jielile, Jiangtao Chen |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Proteomics Pathology medicine.medical_specialty Bioengineering Achilles Tendon Peptide Mapping Applied Microbiology and Biotechnology Biochemistry Protein expression Immobilization Peptide mass fingerprinting Tandem Mass Spectrometry Tendon Injuries Kinesitherapy Animals Humans Cast immobilization Medicine Electrophoresis Gel Two-Dimensional Range of Motion Articular Molecular Biology Polyacrylamide gel electrophoresis Postoperative Care Rupture Achilles tendon business.industry Gene Expression Profiling General Medicine Anatomy Biomechanical Phenomena Casts Surgical medicine.anatomical_structure Models Animal Rabbit model Exercise Movement Techniques Collagen Rabbits Achilles tendon rupture medicine.symptom business Software Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. 165:1092-1106 |
| ISSN: | 1559-0291 0273-2289 |
| DOI: | 10.1007/s12010-011-9327-7 |
| Popis: | Postoperative early kinesitherapy has been advocated as an optimal method for treating Achilles tendon rupture. However, an insight into the rationale of how early kinesitherapy contributes to healing of Achilles tendon remains to be achieved, and research in the area of proteomic analysis of Achilles tendon has so far been lacking. Forty-two rabbits were randomized into control group, immobilization group, and early motion group, and received postoperative cast immobilization and early motion treatments. Achilles tendon samples were prepared 21 days following microsurgery, and the proteins were separated with two-dimensional polyacrylamide gel electrophoresis. Differentially expressed proteins were first recognized by PDQuest software, and then identified using peptide mass fingerprinting, tandem mass spectrometry, and database searching. A total of 463 ± 12, 511 ± 39, and 513 ± 80 protein spots were successfully detected in the two-dimensional polyacrylamide gels for the Achilles tendon samples of rabbits in the control group, immobilization group, and early motion group, respectively. There were 15, 8, and 9 unique proteins in these three groups, respectively, and some differentially expressed proteins were also identified in each group. It was indicated that some of the differentially expressed proteins were involved in various metabolism pathways and may play an important role in healing of Achilles tendon rupture. Postoperative early kinesitherapy resulted in differentially expressed proteins in ruptured Achilles tendon compared with those treated with postoperative cast immobilization. These differentially expressed proteins may contribute to healing of Achilles tendon rupture through a mechanobiological mechanism due to the application of postoperative early kinesitherapy. |
| Databáze: | OpenAIRE |
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