Recent advances in the characterization of Crl, the unconventional activator of the stress sigma factor σS/RpoS
| Autor: | Françoise Norel, Paola Cavaliere |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Salmonella typhimurium
Transcriptional Activation 0301 basic medicine QH301-705.5 sigma s 030106 microbiology salmonella Sigma Factor General Biochemistry Genetics and Molecular Biology Microbiology 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound crl Bacterial Proteins Stress Physiological Sigma factor RNA polymerase Gene expression Escherichia coli Biology (General) Promoter Regions Genetic Transcription factor Polymerase biology Activator (genetics) rna polymerase Gene Expression Regulation Bacterial General Medicine biology.organism_classification rpos Cell biology 030104 developmental biology chemistry Salmonella enterica biology.protein rpoS Protein Binding |
| Zdroj: | Biomolecular Concepts, Vol 7, Iss 3, Pp 197-204 (2016) |
| ISSN: | 1868-5021 |
| Popis: | The bacterial RNA polymerase (RNAP) holoenzyme is a multisubunit core enzyme associated with a σ factor that is required for promoter-specific transcription initiation. Besides a primary σ responsible for most of the gene expression during active growth, bacteria contain alternative σ factors that control adaptive responses. A recurring strategy in the control of σ factor activity is their sequestration by anti-sigma factors that occlude the RNAP binding determinants, reducing their activity. In contrast, the unconventional transcription factor Crl binds specifically to the alternative σ factor σS/RpoS, and favors its association with the core RNAP, thereby increasing its activity. σS is the master regulator of the general stress response that protects many Gram-negative bacteria from several harmful environmental conditions. It is also required for biofilm formation and virulence of Salmonella enterica serovar Typhimurium. In this report, we discuss current knowledge on the regulation and function of Crl in Salmonella and Escherichia coli, two bacterial species in which Crl has been studied. We review recent advances in the structural characterization of the Crl-σS interaction that have led to a better understanding of this unusual mechanism of σ regulation. |
| Databáze: | OpenAIRE |
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