Proteomic Landscape of Extracellular Vesicles for Diffuse Large B-Cell Lymphoma Subtyping
| Autor: | Mostafa Ejtehadifar, Rune Matthiesen, Ana Laura Sousa, Hans Christian Beck, Erin M. Tranfield, José Cabeçadas, Henrique Baeta, Ana Farinho, Maria Gomes da Silva, Paula Gameiro, Andreia Henriques, Ana Sofia Carvalho, Bruno Costa Silva |
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| Přispěvatelé: | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM), Centro de Estudos de Doenças Crónicas (CEDOC), iNOVA4Health - pólo NMS |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
Cell signaling Diffuse large B‐cell lymphoma Proteome QH301-705.5 diffuse large B-cell lymphoma exosomes Biology Exosomes Catalysis Article Mass Spectrometry Inorganic Chemistry Extracellular Vesicles Cell Line Tumor medicine Humans Biology (General) Physical and Theoretical Chemistry KEGG QD1-999 Molecular Biology Spectroscopy B cell B-Lymphocytes Mass spectrometry Organic Chemistry Germinal center General Medicine Extracellular vesicles medicine.disease Germinal Center Microvesicles Computer Science Applications Cell biology Chemistry medicine.anatomical_structure DLBCL Lymphoma Large B-Cell Diffuse Diffuse large B-cell lymphoma |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 20 Carvalho, A S, Baeta, H, Henriques, A F A, Ejtehadifar, M, Tranfield, E M, Sousa, A L, Farinho, A, Silva, B C, Cabeçadas, J, Gameiro, P, da Silva, M G, Beck, H C & Matthiesen, R 2021, ' Proteomic landscape of extracellular vesicles for diffuse large b-cell lymphoma subtyping ', International Journal of Molecular Sciences, vol. 22, no. 20, 11004 . https://doi.org/10.3390/ijms222011004 Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP International Journal of Molecular Sciences, Vol 22, Iss 11004, p 11004 (2021) |
| ISSN: | 1422-0067 |
| Popis: | The role of extracellular vesicles (EVs) proteome in diffuse large B-cell lymphoma (DLBCL) pathology, subclassification, and patient screening is unexplored. We analyzed by state-of-the-art mass spectrometry the whole cell and secreted extracellular vesicles (EVs) proteomes of different molecular subtypes of DLBCL, germinal center B cell (GCB subtype), and activated B cell (ABC subtype). After quality control assessment, we compared whole-cell and secreted EVs proteomes of the two cell-of-origin (COO) categories, GCB and ABC subtypes, resulting in 288/1115 significantly differential expressed proteins from the whole-cell proteome and 228/608 proteins from EVs (adjust p-value < 0.05/p-value < 0.05). In our preclinical model system, we demonstrated that the EV proteome and the whole-cell proteome possess the capacity to separate cell lines into ABC and GCB subtypes. KEGG functional analysis and GO enrichment analysis for cellular component, molecular function, and biological process of differential expressed proteins (DEP) between ABC and GCB EVs showed a significant enrichment of pathways involved in immune response function. Other enriched functional categories for DEPs constitute cellular signaling and intracellular trafficking such as B-cell receptor (BCR), Fc_gamma R-mediated phagocytosis, ErbB signaling, and endocytosis. Our results suggest EVs can be explored as a tool for patient diagnosis, follow-up, and disease monitoring. Finally, this study proposes novel drug targets based on highly expressed proteins, for which antitumor drugs are available suggesting potential combinatorial therapies for aggressive forms of DLBCL. Data are available via ProteomeXchange with identifier PXD028267. |
| Databáze: | OpenAIRE |
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