A potential role for the secretogranin II-derived peptide EM66 in the hypothalamic regulation of feeding behaviour

Autor: Nicolas Chartrel, Youssef Anouar, Julie Maucotel, Jérôme Leprince, Loubna Boukhzar, F. Trebak, Gaëtan Prévost, A. Alaoui, Saloua Cherifi, Céline Duparc, Marie Christine Picot, Arnaud Arabo, Rabia Magoul, Isabelle Dubuc
Přispěvatelé: Neuropsycho-pharmacologie expérimentale, Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), UFR des Sciences et Techniques Mont-Saint-Aignan, University of Bern, Différenciation et communication neuronale et neuroendocrine (DC2N), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Neuroscience Paris Seine (NPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Neuroendocrinologie cellulaire et moléculaire, Les Hôptaux universitaires de Strasbourg (HUS), CHU Strasbourg, Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Centre National d'Études Spatiales [Toulouse] (CNES)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
Endocrinology
Diabetes and Metabolism
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
[SDV]Life Sciences [q-bio]
[CHIM.THER]Chemical Sciences/Medicinal Chemistry
Mice
0302 clinical medicine
Endocrinology
Gene expression
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
MESH: Animals
Receptor
MESH: Peptide Fragments
ComputingMilieux_MISCELLANEOUS
biology
Chemistry
digestive
oral
and skin physiology
Granin
Neuropeptide Y receptor
high-fat diet
Infusions
Intraventricular
Hypothalamus
Secretogranin II
MESH: Feeding Behavior
MESH: Infusions
Intraventricular
hormones
hormone substitutes
and hormone antagonists
medicine.medical_specialty
endocrine system
neuropeptide Y
EM66
Neuropeptide
030209 endocrinology & metabolism
03 medical and health sciences
Cellular and Molecular Neuroscience
Food Preferences
feeding behaviour
Proopiomelanocortin
Arcuate nucleus
MESH: Mice
Inbred C57BL
Internal medicine
medicine
pro-opiomelanocortin
Animals
MESH: Food Preferences
neuropeptide
MESH: Mice
Caloric Restriction
MESH: Caloric Restriction
Endocrine and Autonomic Systems
Appetite Regulation
Feeding Behavior
MESH: Hypothalamus
MESH: Male
Peptide Fragments
Mice
Inbred C57BL
030104 developmental biology
nervous system
biology.protein
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
MESH: Secretogranin II
MESH: Appetite Regulation
Zdroj: Journal of Neuroendocrinology
Journal of Neuroendocrinology, Wiley, 2017, 29 (3), ⟨10.1111/jne.12459⟩
ISSN: 0953-8194
1365-2826
Popis: International audience; EM66 is a conserved 66-amino acid peptide derived from secretogranin II (SgII), a member of the granin protein family. EM66 is widely distributed in secretory granules of endocrine and neuroendocrine cells, as well as in hypothalamic neurones. Although EM66 is abundant in the hypothalamus, its physiological function remains to be determined. The present study aimed to investigate a possible involvement of EM66 in the hypothalamic regulation of feeding behaviour. We show that i.c.v. administration of EM66 induces a drastic dose-dependent inhibition of food intake in mice deprived of food for 18 hours, which is associated with an increase of hypothalamic pro-opiomelanocortin (POMC) and melanocortin-3 receptor mRNA levels and c-Fos immunoreactivity in the POMC neurones of the arcuate nucleus. By contrast, i.c.v. injection of EM66 does not alter the hypothalamic expression of neuropeptide Y (NPY), or that of its Y1 and Y5 receptors. A 3-month high-fat diet (HFD) leads to an important decrease of POMC and SgII mRNA levels in the hypothalamus, whereas NPY gene expression is not affected. Finally, we show that a 48 hours of fasting in HFD mice decreases the expression of POMC and SgII mRNA, which is not observed in mice fed a standard chow. Taken together, the present findings support the view that EM66 is a novel anorexigenic neuropeptide regulating hypothalamic feeding behaviour, at least in part, by activating the POMC neurones of the arcuate nucleus.
Databáze: OpenAIRE
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