Distinctive microRNA expression in early stage nasopharyngeal carcinoma patients
Autor: | Yongming Ma, Chaoyang Wu, Shuna Li, Lihua Hang |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Male Early detection Down-Regulation Biology 03 medical and health sciences 0302 clinical medicine microRNA medicine otorhinolaryngologic diseases Cluster Analysis Humans Stage (cooking) microarrays In Situ Hybridization Aged Neoplasm Staging Oligonucleotide Array Sequence Analysis Nasopharyngeal Carcinoma Gene Expression Profiling Carcinoma Nasopharyngeal Neoplasms Cell Biology Original Articles Pathway enrichment nasopharyngeal carcinomas Middle Aged medicine.disease early stage Molecular biology Tumor formation Up-Regulation Gene expression profiling Gene Expression Regulation Neoplastic stomatognathic diseases MicroRNAs 030104 developmental biology Nasopharyngeal carcinoma Nasopharyngitis 030220 oncology & carcinogenesis Cancer research Molecular Medicine Original Article Female DNA microarray Signal Transduction |
Zdroj: | Journal of Cellular and Molecular Medicine |
ISSN: | 1582-4934 1582-1838 |
Popis: | The goal of this study was to investigate microRNAs (miRs) expression at different stages of nasopharyngeal carcinoma (NPC). MiR expression profiling at various stages of NPC was performed by miR array and further verified using quantitative real‐time RT‐PCR. Pathway enrichment analysis was carried out to identify the functional pathways regulated by the miRs. The expression of a selected group of identified miRs was verified in stage I NPC by in situ hybridization (ISH). A total of 449 miRs were identified with significantly different expressions between NPC tissues and normal pharyngeal tissues. Eighty‐four miRs were dysregulated only in stage I NPC, among which 45 miRs were up‐regulated and the other 39 were down‐regulated. Pathway enrichment assay revleaed that three significantly down‐regulated and three significantly up‐regulated miRs involved in 12 pathways associating with tumour formation and progression. Quantitative RT‐PCR confirmed the miR array result. In addition, the low expression levels of hsa‐miR‐4324, hsa‐miR‐203a and hsa‐miR‐199b‐5p were further validated in stage I NPC by ISH. This present study identifed the miR signature in stage I NPC, providing the basis for early detection and treatment of NPC. |
Databáze: | OpenAIRE |
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