Distinctive microRNA expression in early stage nasopharyngeal carcinoma patients

Autor: Yongming Ma, Chaoyang Wu, Shuna Li, Lihua Hang
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
Early detection
Down-Regulation
Biology
03 medical and health sciences
0302 clinical medicine
microRNA
medicine
otorhinolaryngologic diseases
Cluster Analysis
Humans
Stage (cooking)
microarrays
In Situ Hybridization
Aged
Neoplasm Staging
Oligonucleotide Array Sequence Analysis
Nasopharyngeal Carcinoma
Gene Expression Profiling
Carcinoma
Nasopharyngeal Neoplasms
Cell Biology
Original Articles
Pathway enrichment
nasopharyngeal carcinomas
Middle Aged
medicine.disease
early stage
Molecular biology
Tumor formation
Up-Regulation
Gene expression profiling
Gene Expression Regulation
Neoplastic
stomatognathic diseases
MicroRNAs
030104 developmental biology
Nasopharyngeal carcinoma
Nasopharyngitis
030220 oncology & carcinogenesis
Cancer research
Molecular Medicine
Original Article
Female
DNA microarray
Signal Transduction
Zdroj: Journal of Cellular and Molecular Medicine
ISSN: 1582-4934
1582-1838
Popis: The goal of this study was to investigate microRNAs (miRs) expression at different stages of nasopharyngeal carcinoma (NPC). MiR expression profiling at various stages of NPC was performed by miR array and further verified using quantitative real‐time RT‐PCR. Pathway enrichment analysis was carried out to identify the functional pathways regulated by the miRs. The expression of a selected group of identified miRs was verified in stage I NPC by in situ hybridization (ISH). A total of 449 miRs were identified with significantly different expressions between NPC tissues and normal pharyngeal tissues. Eighty‐four miRs were dysregulated only in stage I NPC, among which 45 miRs were up‐regulated and the other 39 were down‐regulated. Pathway enrichment assay revleaed that three significantly down‐regulated and three significantly up‐regulated miRs involved in 12 pathways associating with tumour formation and progression. Quantitative RT‐PCR confirmed the miR array result. In addition, the low expression levels of hsa‐miR‐4324, hsa‐miR‐203a and hsa‐miR‐199b‐5p were further validated in stage I NPC by ISH. This present study identifed the miR signature in stage I NPC, providing the basis for early detection and treatment of NPC.
Databáze: OpenAIRE
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