Identification of a Huperzine A-producing endophytic fungus from Phlegmariurus taxifolius
| Autor: | Olga Lidia Cruz-Miranda, Jorge Luis Folch-Mallol, Alexandre T. Cardoso Taketa, Fernando Martínez-Morales, Reinier Gesto-Borroto, María Luisa Villarreal |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Fungus Plant use of endophytic fungi in defense Conidium 03 medical and health sciences 0302 clinical medicine Alkaloids Fusarium Botany Genetics medicine Endophytes Lycopodiaceae Internal transcribed spacer DNA Fungal Molecular Biology Huperzine A Mycelium biology General Medicine Huperzia serrata Ribosomal RNA biology.organism_classification 030104 developmental biology 030220 oncology & carcinogenesis Cholinesterase Inhibitors Sesquiterpenes medicine.drug |
| Zdroj: | Molecular biology reports. 47(1) |
| ISSN: | 1573-4978 |
| Popis: | Highly prized huperzine A (Hup A), a natural alkaloid formerly isolated from the Chinese medicinal plant Huperzia serrata, has been widely used for the treatment of Alzheimer disease, inspiring us to search for endophytic fungi that produce this compound. In this study, we obtained the C17 fungus isolate from the Mexican club moss Phlegmariurus taxifolius, which produced a yield of 3.2 μg/g Hup A in mycelial dry weight, when cultured in potato dextrose broth medium. The C17 isolate was identified as belonging to the genus Fusarium with reference to the colony´s morphological characteristics and the presence of macroconidia and microconidia structures; and this was confirmed by DNA-barcoding analysis, by amplifying and sequencing the ribosomal internal transcribed spacer (rITS). |
| Databáze: | OpenAIRE |
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